Bacillus thuringiensis produces the lipopeptide thumolycin to antagonize microbes and nematodes
| Autor: | Ming Sun, Ya-Jie Tang, Lifang Ruan, Bingbing Xue, Yaoyao Deng, Dehong Zheng, Zhiyong Zeng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Insecticides Nematoda 030106 microbiology Bacillus thuringiensis Microbial Sensitivity Tests Biology Microbiology 03 medical and health sciences chemistry.chemical_compound Lipopeptides Biosynthesis Anti-Infective Agents Bacterial Proteins Gene cluster Animals Caenorhabditis elegans Homologous Recombination Pest Control Biological Molecular mass Bacteria Antinematodal Agents Lipopeptide Antimicrobial biology.organism_classification Reverse genetics Molecular Weight chemistry Biochemistry Biological Control Agents Genes Bacterial Multigene Family Mass spectrum analysis Gene Deletion |
| Zdroj: | Microbiological research. 215 |
| ISSN: | 1618-0623 |
| Popis: | Bacillus thuringiensis has been widely used as a bio-insecticide. However, novel biological activities other than insect toxicity of B. thuringiensis are still underestimated. In this study, a new lipopeptide biosynthesis gene cluster in B. thuringiensis BMB171 was discovered by genome mining and verified by reverse genetics. Thumolycin, the lipopeptide synthesized by this gene cluster, was then isolated and purified. Mass spectrum analysis revealed the molecular mass of thumolycin is 696.51 Da with the predicted molecular formula of C38H64N8O4. Further bioactivities assay showed that thumolycin endowed B. thuringiensis BMB171 with broad spectrum antimicrobial and nematocidal activities. |
| Databáze: | OpenAIRE |
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