Follicle-Stimulating Hormone (FSH) Receptors and FSH-Responsive Adenosine 3',5'-Cyclic Monophosphate Production in Porcine Granulosa Cells Decline With Follicular Growth
| Autor: | Andrew R. Labarbera |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
endocrine system
medicine.medical_specialty Swine medicine.drug_class Ovary Biology Iodine Radioisotopes Adenylyl cyclase Follicle-stimulating hormone chemistry.chemical_compound Endocrinology Ovarian Follicle 1-Methyl-3-isobutylxanthine Internal medicine Follicular phase Cyclic AMP medicine Animals Receptor Granulosa Cells Temperature General Medicine Ligand (biochemistry) Culture Media Kinetics Chemically defined medium medicine.anatomical_structure chemistry Receptors FSH Female Follicle Stimulating Hormone Gonadotropin Adenylyl Cyclases |
| Zdroj: | Endocrine Research. 20:65-77 |
| ISSN: | 1532-4206 0743-5800 |
| DOI: | 10.1080/07435809409035857 |
| Popis: | Comparison of binding of 125I-porcine FSH to porcine granulosa cells from small and large ovarian follicles indicated that binding is dependent on both time and temperature. At 37 C, binding of ligand is more than four times higher in cells from small follicles than in cells from large follicles within 30 minutes of the start of incubation. Binding to cells from both small and large follicles is more stable for a longer period of time at 20 C than at 37 C. Equilibrium saturation binding analysis of 125I-pFSH binding indicated that binding is saturable and of high affinity. Granulosa cells from small and large follicles have similar affinities for 125I-pFSH. However, granulosa cells from small follicles have 4.7-times as many FSH receptors as granulosa cells from large follicles. The sensitivity and responsiveness of the adenylyl cyclase system to FSH were assessed by incubating cells from small and large follicles with increasing concentrations of FSH in a defined medium containing isobutylmethylxanthine (1 mM), an inhibitor of phosphodiesterase. Granulosa cells from small follicles are more sensitive to FSH and have a greater cAMP response to FSH than granulosa cells from large follicles. Thus, both active FSH receptors and FSH-responsive adenylyl cyclase activity decline during follicular growth. |
| Databáze: | OpenAIRE |
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