Oxidative and nitrosative stress responses during macrophage-Candida albicans biofilm interaction
| Autor: | Julio E Arce Miranda, Claudia E Sotomayor, José L. Baronetti, M Gabriela Paraje |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Antifungal Agents
Oxidative phosphorylation medicine.disease_cause Models Biological Antioxidants Microbiology 03 medical and health sciences Mice Immune system Drug Resistance Fungal Candida albicans medicine Animals 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species Innate immune system biology 030306 microbiology Macrophages Biofilm General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Reactive Nitrogen Species Corpus albicans Oxidative Stress Infectious Diseases RAW 264.7 Cells chemistry Nitrosative Stress Biofilms Host-Pathogen Interactions Mutation Reactive Oxygen Species Oxidative stress |
| Zdroj: | Medical mycology. 57(1) |
| ISSN: | 1460-2709 |
| Popis: | Candida albicans is an important source of device-associated infection because of its capacity for biofilm formation. This yeast has the ability to form biofilms which favors the persistence of the infection. Furthermore, the innate immune response has a critical role in the control of these infections and macrophages (Mo) are vital to this process. An important fungicidal mechanism employed by Mo involves the generation of toxic reactive oxygen species (ROS) and reactive nitrogen intermediates (RNI). The interaction between biofilms and these immune cells, and the contribution of oxidative and nitrosative stress, that is determinant to the course of the infection, remains elusive. The aim of this study was to investigate this interaction. To this purpose, two models of Mo-biofilms contact, early (model 1) and mature (model 2) biofilms, were used; and the production of ROS, RNI and the oxidative stress response (OSR) were evaluated. We found that the presence of Mo decreased the biofilm formation at an early stage and increased the production of ROS and RNI, with activation of ORS (enzymatic and nonenzymatic). On the other hand, the interaction between mature biofilms and Mo resulted in an increasing biofilm formation, with low levels of RNI and ROS production and decrease of OSR. Dynamic interactions between Mo and fungal biofilms were also clearly evident from images obtained by confocal scanning laser microscopy. The prooxidant-antioxidant balance was different depending of C. albicans biofilms stages and likely acts as a signal over their formation in presence of Mo. These results may contribute to a better understanding of the immune-pathogenesis of C. albicans biofilm infections. |
| Databáze: | OpenAIRE |
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