A Recombinant Alpha-Like Protein Subunit Vaccine (GBS-NN) Provides Protection in Murine Models of Group B Streptococcus Infection
| Autor: | Brokaw, Alyssa, Nguyen, Shayla, Quach, Phoenicia, Orvis, Austyn, Furuta, Anna, Johansson-Lindbom, Bengt, Fischer, Per B, Rajagopal, Lakshmi |
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| Rok vydání: | 2022 |
| Předmět: |
Adult
Streptococcal Vaccines Streptococcus bacterial infections and mycoses Streptococcus agalactiae Disease Models Animal Mice Protein Subunits Infectious Diseases Pregnancy Streptococcal Infections Vaccines Subunit Major Article Animals Humans Immunology and Allergy Female Pregnancy Complications Infectious reproductive and urinary physiology |
| Zdroj: | J Infect Dis |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Background Group B Streptococcus (GBS) transmission during pregnancy causes preterm labor, stillbirths, fetal injury, or neonatal infections. Rates of adult infections are also rising. The GBS-NN vaccine, engineered by fusing N-terminal domains of GBS Alpha C and Rib proteins, is safe in healthy, nonpregnant women, but further assessment is needed for use during pregnancy. Here, we tested GBS-NN vaccine efficacy using mouse models that recapitulate human GBS infection outcomes. Methods Following administration of GBS-NN vaccine or adjuvant, antibody profiles were compared by ELISA. Vaccine efficacy was examined by comparing infection outcomes in GBS-NN vaccinated versus adjuvant controls during systemic and pregnancy-associated infections, and during intranasal infection of neonatal mice following maternal vaccination. Results Vaccinated mice had higher GBS-NN–specific IgG titers versus controls. These antibodies bound alpha C and Rib on GBS clinical isolates. Fewer GBS were recovered from systemically challenged vaccinated mice versus controls. Although vaccination did not eliminate GBS during ascending infection in pregnancy, vaccinated dams experienced fewer in utero fetal deaths. Additionally, maternal vaccination prolonged neonatal survival following intranasal GBS challenge. Conclusions These findings demonstrate GBS-NN vaccine efficacy in murine systemic and perinatal GBS infections and suggest that maternal vaccination facilitates the transfer of protective antibodies to neonates. |
| Databáze: | OpenAIRE |
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