Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein–Protein Interactions in Plant Cells
| Autor: | Kazumi Hikino, Shoji Mano, Tsuyoshi Nakagawa, Mikio Nishimura, Shino Goto-Yamada |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agroinfiltration Chemistry Plant cell 01 natural sciences Protein–protein interaction Fusion gene 03 medical and health sciences Bimolecular fluorescence complementation 030104 developmental biology Biophysics Fluorescent protein Gene 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493978700 |
| DOI: | 10.1007/978-1-4939-7871-7_16 |
| Popis: | The bimolecular fluorescence complementation (BiFC) assay is a powerful, flexible, and simple tool to study protein-protein interactions in living cells. To accelerate the production and assessment of BiFC constructs, Gateway-compatible multicolor BiFC vectors were generated to enable the simultaneous production of multiple fusion genes that have the split N- or C-terminal fragment of fluorescent protein with the gene of interest in a high-throughput manner. Two different transient expression techniques for the assessment of BiFC in plant cells are described. |
| Databáze: | OpenAIRE |
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