Effect of liposome-encapsulated hemoglobin on triglyceride, total cholesterol, low-density lipoprotein, and high-density lipoprotein cholesterol measurements
| Autor: | M. Whiteford, Alan S. Rudolph, Philip Ovadia, Reuven Rabinovici, Fizan Abdullah, L. F. Neville, Guenther Mathiak |
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| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Very low-density lipoprotein Clinical chemistry In Vitro Techniques Biochemistry Rats Sprague-Dawley Hemoglobins chemistry.chemical_compound High-density lipoprotein Blood Substitutes Internal medicine medicine Animals Infusions Intravenous Triglycerides Cholesterol Measurement Triglyceride Cholesterol Cholesterol HDL Organic Chemistry Cholesterol LDL Cell Biology Rats Endocrinology chemistry Low-density lipoprotein Liposomes lipids (amino acids peptides and proteins) Hemoglobin |
| Zdroj: | Lipids. 32:377-381 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/s11745-997-0048-6 |
| Popis: | The present study investigated the effect of liposome-encapsulated hemoglobin (LEH), an experimental oxygen-carrying resuscitation fluid, on triglyceride, total cholesterol, and low density lipoprotein (LDL), and high density lipoprotein (HDL) cholesterol measurements. In vivo, the intravenous infusion of LEH (5.6 mL/kg, n = 6) elevated serum triglycerides (+92% vs. baseline, P.05), total cholesterol (+25% vs. baseline, P.01), LDL cholesterol (+72% vs. baseline, P.01) and had no effect on serum HDL cholesterol. In addition, LEH did not alter the elevation in serum triglycerides (+302% vs. baseline, P.01) and LDL cholesterol (+86% vs. baseline, P.01) induced by lipopolysaccharide (3.6 mg/kg, i.v., n = 6. Ex vivo, measurements of triglycerides and total cholesterol as well as LDL and HDL cholesterol in whole blood from naive rats were not changed by the addition of LEH (0-50%, n = 6). In vitro, the addition of a fixed concentration of LEH (50%, n = 6) to varying concentrations of cholesterol solution (0-50%), or vice versa, had no effect on cholesterol determination. It is therefore concluded that LEH only minimally affects serum levels of triglycerides, total cholesterol, LDL cholesterol, and HDL cholesterol and does not interfere with their measurement. |
| Databáze: | OpenAIRE |
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