Implication of sphingolipid metabolism in the stability of the Golgi apparatus
| Autor: | Akitsugu Yamamoto, Mitsuo Tagaya, Kiyotaka Hatsuzawa, Takuya Fukunaga, Masami Nagahama, Katsuko Tani |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Endosome
Morpholines Golgi Apparatus Oleic Acids Endosomes Biology Ceramides Cell Line Coat Protein Complex I chemistry.chemical_compound symbols.namesake Sphingosine Animals Masoprocol Coat Proteins Enzyme Inhibitors Sphingolipids Brefeldin A Nocodazole Cell Biology Golgi apparatus Cell biology Nordihydroguaiaretic acid Microscopy Electron Sphingomyelin Phosphodiesterase chemistry Ethanolamines Golgi disassembly Sphingolipid metabolism symbols Cattle Sphingomyelin Endocannabinoids trans-Golgi Network |
| Zdroj: | Journal of Cell Science. 113:3299-3307 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.113.18.3299 |
| Popis: | We examined the effects of short chain and long chain ceramides on the stability of the Golgi apparatus. Short chain ceramides, C(2)- and C(6)-ceramides, blocked brefeldin A-induced Golgi disassembly without affecting the rapid release of Golgi coat proteins, whereas they did not inhibit brefeldin A-induced tubulation of endosomes. Both short chain ceramides also retarded Golgi disassembly induced by nordihydroguaiaretic acid and nocodazole, suggesting that they stabilize the Golgi apparatus. In contrast to short chain ceramides, natural long chain ceramides, when incorporated into cells or formed within cells upon treatment with sphingomyelinase or metabolic inhibitors, enhanced brefeldin A-induced Golgi disassembly. These results suggest that sphingolipid metabolism is implicated in the stability of the Golgi apparatus. |
| Databáze: | OpenAIRE |
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