Exo‐inulinase production from Aspergillus fumigatus NFCCI 2426: purification, characterization, and immobilization for continuous fructose production
| Autor: | Ritumbhara Choukade, Bhanu Pratap Prajapati, Hemant Kumar Rawat, Naveen Kango, Rahul Kumar Suryawanshi, Hemant Soni |
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| Rok vydání: | 2021 |
| Předmět: |
Sucrose
Calcium alginate Glycoside Hydrolases Taraxacum 030309 nutrition & dietetics Dandelion Fructose Plant Roots Chicory Aspergillus fumigatus 03 medical and health sciences chemistry.chemical_compound Hydrolysis 0404 agricultural biotechnology Inulinase 0303 health sciences Chromatography biology Chemistry Inulin food and beverages 04 agricultural and veterinary sciences Enzymes Immobilized biology.organism_classification 040401 food science Glutaraldehyde Food Science |
| Zdroj: | Journal of Food Science. 86:1778-1790 |
| ISSN: | 1750-3841 0022-1147 |
| Popis: | Aspergillus fumigatus was found to produce thermostable exo-inulinase (EC 3.8.1.80; 38 U/ml) on inulin-rich infusions. Exo-inulinase (14.6 U/mg) was immobilized on glutaraldehyde activated Ca-alginate beads for continuous generation of fructose by hydrolyzing sucrose, chicory, and dandelion substrates. Immobilization of enzyme was confirmed by microscopic and spectroscopic techniques. The exo-inulinase was purified using ion-exchange (1.30-folds) and size-exclusion chromatography (2.71-folds). The purified exo-inulinase showed 64 kDa band on gel and was optimally active at 60 °C and pH 6.0. Kinetic constants, Km and Vmax of purified exo-inulinase, were 5.88 mM and 1.66 µM/min, respectively, and its relative activity was found to be enhanced (125.8%) in the presence of calcium ion. Immobilized preparation was utilized for continuous generation of fructose from chicory juice (26 to 70%) and dandelion root extracts (16 to 24%) by recycling upto five cycles, respectively. In comparison to other sweeteners, such as sucrose, fructose is considered as a healthy alternative. The present study demonstrated the use of immobilized exo-inulinase in continuous generation of fructose from some underutilized plant sources that can be used in food industry. PRACTICAL APPLICATION: Thermostable exo-inulinase produced by A. fumigatus was immobilized on calcium alginate matrix and was employed for continuous hydrolysis of chicory juice and dandelion root extract for generation of fructose syrup. |
| Databáze: | OpenAIRE |
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