Sabinene Prevents Skeletal Muscle Atrophy by Inhibiting the MAPK–MuRF-1 Pathway in Rats

Autor: Su Jung Kim, Hengzhe Jin, Donghyen Lee, Kyung-Jin Lee, Yunkyoung Ryu, Kyung-Jong Won, Hwan Myung Lee, Bokyung Kim, Seung Hyo Jung
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
MAPK/ERK pathway
Muscle Proteins
Myoblasts
Tripartite Motif Proteins
lcsh:Chemistry
0302 clinical medicine
Myocyte
lcsh:QH301-705.5
Spectroscopy
Bicyclic Monoterpenes
chemistry.chemical_classification
biology
chrysanthemum boreale makino essential oil
Kinase
Chemistry
Myogenesis
Chrysanthemum boreale Makino essential oil
General Medicine
Muscle atrophy
Computer Science Applications
Ubiquitin ligase
Muscular Atrophy
Mitogen-Activated Protein Kinases
medicine.symptom
Signal Transduction
medicine.medical_specialty
Cell Survival
Ubiquitin-Protein Ligases
sabinene
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
skeletal muscle atrophy
Internal medicine
medicine
Animals
Physical and Theoretical Chemistry
Muscle
Skeletal
Protein kinase A
Molecular Biology
Reactive oxygen species
Organic Chemistry
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
mapk/murf-1
biology.protein
l6 cells
Reactive Oxygen Species
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 19, p 4955 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 19
ISSN: 1422-0067
Popis: Chrysanthemum boreale Makino essential oil (CBMEO) has diverse biological activities including a skin regenerating effect. However, its role in muscle atrophy remains unknown. This study explored the effects of CBMEO and its active ingredients on skeletal muscle atrophy using in vitro and in vivo models of muscle atrophy. CBMEO reversed the size decrease of L6 myoblasts under starvation. Among the eight monoterpene compounds of CBMEO without cytotoxicity for L6 cells, sabinene induced predominant recovery of reductions of myotube diameters under starvation. Sabinene diminished the elevated E3 ubiquitin ligase muscle ring-finger protein-1 (MuRF-1) expression and p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylations in starved myotubes. Moreover, sabinene decreased the increased level of reactive oxygen species (ROS) in myotubes under starvation. The ROS inhibitor antagonized expression of MuRF-1 and phosphorylation of MAPKs, which were elevated in starved myotubes. In addition, levels of muscle fiber atrophy and MuRF-1 expression in gastrocnemius from fasted rats were reduced after administration of sabinene. These findings demonstrate that sabinene, a bioactive component from CBMEO, may attenuate skeletal muscle atrophy by regulating the activation mechanism of ROS-mediated MAPK/MuRF-1 pathways in starved myotubes, probably leading to the reverse of reduced muscle fiber size in fasted rats.
Databáze: OpenAIRE
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