Nitric Oxide Modulates the Synthesis of Extracellular Matrix Proteins in Cultured Rat Mesangial Cells
| Autor: | Howard Trachtman, Stephen Futterweit, Pravin C. Singhal |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Biophysics Matrix (biology) Arginine Nitric Oxide Biochemistry Nitric oxide Extracellular matrix Interferon-gamma chemistry.chemical_compound Laminin Animals Molecular Biology Cells Cultured Nitrites Extracellular Matrix Proteins biology Mesangial cell Chemistry Cell Biology Recombinant Proteins Fibronectins Glomerular Mesangium Rats Cell biology Fibronectin Nitric oxide synthase NG-Nitroarginine Methyl Ester biology.protein Collagen |
| Zdroj: | Biochemical and Biophysical Research Communications. 207:120-125 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1995.1161 |
| Popis: | Nitric oxide (NO) is an important effector molecule of the inflammatory response. It is synthesized by mesangial cells and has been proposed to contribute to glomerular injury in various disease states. We studied whether NO modulates extracellular matrix production in cultured rat mesangial cells. Stimulation of rat mesangial cell NO release with gamma-interferon and lipopolysaccharide resulted in reduced production of collagen (by 35%) fibronectin (by 48%) (P0.05). In contrast, laminin synthesis was enhanced two-fold by the same maneuver (P0.05). These changes were reversed by the addition of L-NAME, a selective inhibitor of inducible nitric oxide synthase. This is the first demonstration that NO regulates the synthesis of extracellular matrix by mesangial cells. The results indicate that increased renal production of NO in glomerular diseases may attenuate the production and accumulation of matrix proteins and limit the severity of glomerulosclerosis. |
| Databáze: | OpenAIRE |
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