Enterobacterial intergenic consensus sequence polymerase chain reaction as a typing method for Burkholderia (Pseudomonas) cepacia
| Autor: | Nevio Cimolai, Colleen Trombley |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
biology Inverse polymerase chain reaction polymerase chain reaction General Medicine Computational biology Burkholderia cepacia biology.organism_classification Microbiology law.invention Burkholderia Infectious Diseases law Primer dimer Agarose gel electrophoresis epidemiology Typing Primer (molecular biology) Polymerase chain reaction In silico PCR |
| Zdroj: | Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 2(1) |
| ISSN: | 1469-0691 |
| Popis: | Objective We developed a rapid polymerase chain reaction (PCR) method, which utilizes primers from an entero-bacterial repetitive consensus sequence, to differentiate among strains of Burkholderia cepacia . We then applied this method to isolates from a device-associated pseudoinfection and also compared the discriminative typing potential with that of another PCR-based method. Methods A simplified lysis procedure was used to generate DNA substrate for amplification by a PCR method which utilized primer ERIC2 and a reverse complement of ERIC1R. The alternative PCR-based method employed primers which are directed to Bordetella pertussis repetitive sequences. Amplification products were visually assessed after conventional agarose gel electrophoresis and staining. Results The ERIC-based method appropriately clustered strains for the device-associated pseudo-infection. In addition, there was consistency in the definition of similar or dissimilar strains between the two PCR-based systems. Conclusions The ERIC-based PCR typing method offers sufficient discriminatory power for it to be used for epidemic-logic purposes. |
| Databáze: | OpenAIRE |
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