A Single Spherical Assembly of Protein Amyloid Fibrils Formed by Laser Trapping
| Autor: | Shun Hirota, Ken-ichi Yuyama, Teruki Sugiyama, Mariko Ueda, Satoshi Nagao, Hiroshi Masuhara |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Amyloid nucleation Dimer Nucleation Nanotechnology 02 engineering and technology Catalysis law.invention chemistry.chemical_compound Laser trapping 03 medical and health sciences spatio-temporal formation law mental disorders laser trapping amyloids domain-swapped protein General Chemistry General Medicine Microstructure 021001 nanoscience & nanotechnology Fluorescence Characterization (materials science) 030104 developmental biology chemistry Biophysics Electron microscope 0210 nano-technology |
| Zdroj: | Angewandte Chemie. 129:6843-6847 |
| ISSN: | 0044-8249 |
| Popis: | Protein amyloids have received much attention owing to their correlation with serious diseases and to their promising mechanical and optical properties as future materials. Amyloid formation has been conducted by tuning temperature and chemical conditions, so that its nucleation and the following growth are analyzed as ensemble dynamics. A single spherical assembly of amyloid fibrils of cytochrome c domain-swapped dimer was successfully generated upon laser trapping. The amyloid fibrillar structure was confirmed by fluorescence characterization and electron microscopy. The prepared spheres were further manipulated individually in solution to fabricate a three-dimensional microstructure and a line pattern. Amyloid formation dynamics and amyloid-based microstructure fabrication are demonstrated based on direct observation of a single spherical assembly, which foresees a new approach in amyloid studies. |
| Databáze: | OpenAIRE |
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