Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus
| Autor: | J Needham, Hagen Scherb, Esther Mahabir, Jörg Schmidt, D Bulian, K. Reindl, Kyriaki Markoullis, Josef Mysliwietz |
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| Rok vydání: | 2008 |
| Předmět: |
Male
viruses Germline transmission Fertilization in Vitro Virus Replication Virus Parvoviridae Infections Mice Mouse hepatitis virus Seroepidemiologic Studies Genetics medicine Animals Blastocyst Embryonic Stem Cells Original Paper Mice Inbred ICR Murine hepatitis virus biology virus diseases Embryo Embryo Transfer Flow Cytometry biology.organism_classification Embryonic stem cell Virology Mice Inbred C57BL Murine embryonic stem cells Germ Cells medicine.anatomical_structure Viral replication Hepatitis Viral Animal Minute Virus of Mice Receptors Virus Female Animal Science and Zoology Mouse minute virus Stem cell Coronavirus Infections Agronomy and Crop Science Minute virus of mice Biotechnology |
| Zdroj: | Transgenic Research |
| ISSN: | 1573-9368 0962-8819 |
| Popis: | The aim of this study was to determine the susceptibility of murine embryonic stem (mESCs) to mouse hepatitis virus (MHV-A59) and mouse minute virus (MMVp) and the effect of these viruses on germline transmission (GLT) and the serological status of recipients and pups. When recipients received 10 blastocysts, each injected with 10(0) TCID(50) MHV-A59, three out of five recipients and four out of 14 pups from three litters became seropositive. When blastocysts were injected with 10(-5) TCID(50) MMVp, all four recipients and 14 pups from four litters remained seronegative. The mESCs replicated MHV-A59 but not MMVp, MHV-A59 being cytolytic for mESCs. Exposure of mESCs to the viruses over four to five passages but not for 6 h affected GLT. Recipients were seropositive for MHV-A59 but not for MMVp when mESCs were cultured with the virus over four or five passages. The data show that GLT is affected by virus-contaminated mESCs. |
| Databáze: | OpenAIRE |
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