Protein Profile and Morphological Alterations in Penumbra after Focal Photothrombotic Infarction in the Rat Cerebral Cortex
| Autor: | Svetlana Demyanenko, Anatoly B. Uzdensky, Alexej Fedorenko, Tayana Lapteva, Grigory Fedorenko |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Brain Infarction Male Proteomics Neurofilament Doublecortin Protein Light Neuroscience (miscellaneous) Cell Count Biology Neuroprotection 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine Animals Rats Wistar Protein kinase A Protein kinase C Cerebral Cortex Neurons Tyrosine hydroxylase Penumbra Neurodegeneration Thrombosis Somatosensory Cortex medicine.disease Cell biology Doublecortin 030104 developmental biology Neurology biology.protein Neuroscience 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Molecular neurobiology. 54(6) |
| ISSN: | 1559-1182 |
| Popis: | After ischemic stroke, cell damage propagates from infarct core to surrounding tissues (penumbra). To reveal proteins involved in neurodegeneration and neuroprotection in penumbra, we studied protein expression changes in 2-mm ring around the core of photothrombotic infarct induced in the rat brain cortex by local laser irradiation after administration of Bengal Rose. The ultrastructural study showed edema and degeneration of neurons, glia, and capillaries. Morphological changes gradually decreased across the penumbra. Using the antibody microarrays, we studied changes in expression of >200 neuronal proteins in penumbra 4 or 24 h after focal photothrombotic infarct. Diverse cellular subsystems were involved in the penumbra tissue response: signal transduction pathways such as protein kinase Bα/GSK-3, protein kinase C and its β1 and β2 isoforms, Wnt/β-catenin (axin1, GSK-3, FRAT1), Notch/NUMB, DYRK1A, TDP43; mitochondria quality control (Pink1, parkin, HtrA2); ubiquitin-mediated proteolysis (ubiquilin-1, UCHL1); axon outgrowth and guidance (NAV-3, CRMP2, PKCβ2); vesicular trafficking (syntaxin-8, TMP21, Munc-18-3, synip, ALS2, VILIP1, syntaxin, synaptophysin, synaptotagmin); biosynthesis of neuromediators (tryptophan hydroxylase, monoamine oxidase B, glutamate decarboxylase, tyrosine hydroxylase, DOPA decarboxylase, dopamine transporter); intercellular interactions (N-cadherin, PMP22); cytoskeleton (neurofilament 68, neurofilament-M, doublecortin); and other proteins (LRP1, prion protein, β-amyloid). These proteins are involved in neurodegeneration or neuroprotection. Such changes were most expressed 4 h after photothrombotic impact. Immunohistochemical and Western blot studies of expression of monoamine oxidase B, UCHL1, DYRK1A, and Munc-18-3 confirmed the proteomic data. These data provide the integral view on the penumbra response to photothrombotic infarct. Some of these proteins can be potential targets for ischemic stroke therapy. |
| Databáze: | OpenAIRE |
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