Effect of mild heat stress on heat shock protein 70 in a balneotherapy model
| Autor: | Emel Uzunoğlu, Sibel P. Yentür, Ali Hikmet Kayar, Nergis Erdoğan, Guher Saruhan Direskeneli, Arif Dönmez, Mustafa Turan |
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| Přispěvatelé: | Belirlenecek, TOBB ETU, Faculty of Medicine, Department of Basic Medical Sciences, TOBB ETÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Turan, Mustafa, U-5499-2017 |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
030203 arthritis & rheumatology
0301 basic medicine Balneotherapy Heat shock protein business.industry ELISPOT medicine.medical_treatment Thermal water Stimulation Peripheral blood mononuclear cell Hsp70 Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Immune system Complementary and alternative medicine Immunology Osteoarthritis Medicine Heat shock business |
| Popis: | Donmez, Arif/0000-0002-7241-075X; Turan, Mustafa/0000-0003-4782-8596 WOS: 000396403400012 Introduction: Fever-induced mild heat stress plays an important role in cellular responses. The best-known heat shock response is heat shock protein (HSP) secretion. We investigated the effect of mild heat stress on serum HSP70 levels and a possible association between the immune system and these molecules by applying a mild heat stress model using a thermal water bath. Method: Peripheral blood samples from 16 osteoarthritis (OA) patients who were treated with 39-40 degrees C thermo-mineral water immersion were examined. Samples were drawn before the first bath, after the first bath, and at the end of treatment. HSP70 levels and interferon-gamma (IFN)-gamma responses in peripheral blood mononuclear cells (PBMC) were evaluated in plasma by enzyme-linked immunosorbent assay ( ELISA) and enzyme-linked immunosorbent spot (ELISPOT) assay, respectively. Results: IFN-gamma-secreting PBMC numbers were increased significantly in response to HSP70, purified protein derivative (PPD), and phytohemagglutinin (PHA) stimulation after the first bath, as compared to before bathing values (p=0.005, p=0.012, p=0.021). HSP level in serum was also increased after the first bath, and decreased to near-baseline levels after treatment, however the differences were not significant. Conclusions: The initial increase in HSP 70 and IFN-gamma levels after the first bath and the final decrease at the end of serial baths suggest that the immune system adapts to the heat stress at the end of this intervention. (C) 2016 Elsevier GmbH. All rights reserved. Istanbul University Scientific Research UnitIstanbul University [213/29042004] This work was supported by Istanbul University Scientific Research Unit (Project No. 213/29042004). |
| Databáze: | OpenAIRE |
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