Reduced expression of the complement receptor type 2 (CR2, CD21) by synovial fluid B and T lymphocytes
| Autor: | Harald Illges, I. Melchers, Hans-Hartmut Peter, M. Braun |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
T-Lymphocytes Cellular differentiation Immunology Gene Expression Arthritis chemical and pharmacologic phenomena Complement receptor Arthritis Reactive Arthritis Rheumatoid TCIRG1 Immune system immune system diseases hemic and lymphatic diseases Synovial Fluid medicine Humans Immunology and Allergy Synovial fluid Spondylitis Ankylosing RNA Messenger DNA Primers B-Lymphocytes Base Sequence business.industry Arthritis Psoriatic hemic and immune systems T lymphocyte medicine.disease medicine.anatomical_structure Case-Control Studies Lung Immunology/Disease Receptors Complement 3d Synovial membrane business Still's Disease Adult-Onset |
| Zdroj: | Clinical and Experimental Immunology. 122:270-276 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.2000.01379.x |
| Popis: | SUMMARYThe expression of CR2 (CD21) by synovial B and T lymphocytes of patients suffering from various forms of arthritis was analysed with cytofluorometry and with reverse transcriptase-polymerase chain reaction. CR2 (CD21) cell surface protein was detected in normal quantities on peripheral B cells, but was almost absent on synovial B lymphocytes of the same patients. This reduction was most severe in patients with rheumatoid arthritis, but also observed in all other cases. CR2 (CD21) did not reappear after in vitro culture. CR2 (CD21) mRNA was also strongly reduced in synovial B and T lymphocytes. Synovial fluid B lymphocytes were larger than peripheral blood B lymphocytes, while T cells from the same patients showed no size differences. We conclude that synovial fluid B lymphocytes have undergone an irreversible step towards terminal differentiation. The presence or absence of CR2 (CD21) mRNA in peripheral versus synovial T cells indicates that CR2 (CD21) is also differentially expressed by T lymphocytes. |
| Databáze: | OpenAIRE |
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