Targeted nanobubbles in low-frequency ultrasound-mediated gene transfection and growth inhibition of hepatocellular carcinoma cells
| Autor: | Hui Jing, Xue Han, Bolin Wu, Wen Cheng, Hao Zhang, Qiang Qiao, Hongjian Liang |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Small interfering RNA Carcinoma Hepatocellular Apoptosis Biology Transfection Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine RNA interference Cell Movement medicine Gene silencing Humans Neoplasm Invasiveness Ultrasonics RNA Small Interfering Cell Proliferation Oncogene Proteins Messenger RNA Microbubbles medicine.diagnostic_test Liver Neoplasms General Medicine Genetic Therapy medicine.disease Molecular biology 030104 developmental biology chemistry 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research Growth inhibition CRISPR-Cas Systems |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 37(9) |
| ISSN: | 1423-0380 |
| Popis: | The use of SonoVue combined with ultrasound exposure increases the transfection efficiency of short interfering RNA (siRNA). The objective of this study was to prepare targeted nanobubbles (TNB) conjugated with NET-1 siRNA and an antibody GPC3 to direct nanobubbles to hepatocellular carcinoma cells. SMMC-7721 human hepatocellular carcinoma cells were treated with six different groups. The transfection efficiency and cellular apoptosis were measured by flow cytometry. The protein and messenger RNA (mRNA) expression were measured by Western blot and quantitative real-time PCR, respectively. The migration and invasion potential of the cells were determined by Transwell analysis. The results show that US-guided siRNA-TNB transfection effectively enhanced gene silencing. In summary, siRNA-TNB may be an effective delivery vector to mediate highly effective RNA interference in tumor treatment. |
| Databáze: | OpenAIRE |
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