Assessment of Vibrio parahaemolyticus levels in oysters (Crassostrea virginica) and seawater in Delaware Bay in relation to environmental conditions and the prevalence of molecular markers to identify pathogenic Vibrio parahaemolyticus strains

Autor: Gulnihal Ozbay, Amanda Abbott, Esam Almuhaideb, Lathadevi K. Chintapenta, Salina Parveen
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Oyster
Salinity
Marine and Aquatic Sciences
Oceanography
Physical Chemistry
Biochemistry
Oysters
Hemolysin Proteins
Water Quality
0303 health sciences
Multidisciplinary
biology
Vibrio parahaemolyticus
food and beverages
Eukaryota
Hemolysin
Proteases
Enzymes
Chemistry
Bays
Physical Sciences
Crassostrea
Medicine
Research Article
Environmental Monitoring
Bivalves
Science
Microbiology
03 medical and health sciences
Sea Water
biology.animal
Genetics
Animals
Humans
Seawater
Dissolved Oxygen
Thermolabile
Ocean Temperature
030304 developmental biology
Vibrio
Bacteria
030306 microbiology
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Aquatic Environments
Proteins
Molluscs
biology.organism_classification
equipment and supplies
Delaware
Invertebrates
Marine Environments
Ostreidae
Chemical Properties
Seafood
Genetic marker
Earth Sciences
Enzymology
Metalloproteases
bacteria
Zoology
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 12, p e0242229 (2020)
ISSN: 1932-6203
Popis: This study identifiedVibrio parahaemolyticusin oyster and seawater samples collected from Delaware Bay from June through October of 2016. Environmental parameters including water temperature, salinity, dissolved oxygen, pH, and chlorophyllawere measured per sampling event. Oysters homogenate and seawater samples were 10-fold serially diluted and directly plated on CHROMagarᵀᴹ Vibrio medium. PresumptiveV.parahaemolyticuscolonies were counted and at least 20% of these colonies were selected for molecular chracterization.V.parahaemolyticusisolates (n = 165) were screened for the presence of the species-specific thermolabile hemolysin (tlh) gene, the pathogenic thermostable direct hemolysin (tdh)/ thermostable related hemolysin (trh) genes, the regulatory transmembrane DNA-binding gene (toxR), andV.parahaemolyticusmetalloprotease (vpm) gene using a conventional PCR. The highest mean levels of the presumptiveV.parahaemolyticuswere 9.63×103CFU/g and 1.85×103CFU/mL in the oyster and seawater samples, respectively, during the month of July.V.parahaemolyticuslevels in oyster and seawater samples were significantly positively correlated with water temperature. Of the 165 isolates, 137 (83%), 110 (66.7%), and 108 (65%) weretlh+,vpm+, andtoxR+, respectively. Among theV.parahaemolyticus(tlh+) isolates, 7 (5.1%) and 15 (10.9%) weretdh+andtrh+, respectively, and 24 (17.5%), only oyster isolates, were positive for both genes. Potential pathogenic strains that possessedtdhand/ortrhwere notably higher in oyster (39%) than seawater (15.6%) isolates. The occurrence of totalV.parahaemolyticus(tlh+) was not necessarily proportional to the potential pathogenicV.parahaemolyticus. Co-occurrence of the five genetic markers were observed only among oyster isolates. The co-occurrence of the gene markers showed a relatedness potential oftdhoccurrence withvpm. We believe exploring the role ofV.parahaemolyticusmetalloprotease and whether it is involved in the toxic activity of the thermostable direct hemolysin (TDH) protein can be of significance. The outcomes of this study will provide some foundation for future studies regarding pathogenicVibriodynamics in relation to environmental quality.
Databáze: OpenAIRE
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