Potential role of leptin in increase of fatty acid synthase gene expression in chicken liver

Autor: Johan Buyse, Mohammed Taouis, Sami Dridi, Eddy Decuypere
Přispěvatelé: ProdInra, Migration, Laboratory of Physiology, Immunology, and Genetics of Domestic Animals, Catholic University of Leuven, Laboratoire de Neuroendocrinologie Moléculaire de la Prise Alimentaire, Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Université Catholique de Louvain = Catholic University of Louvain (UCL), Neurobiologie de l'Olfaction et de la Prise Alimentaire (NOPA), Institut National de la Recherche Agronomique (INRA), Université Paris-Sud - Paris 11 (UP11)
Rok vydání: 2005
Předmět:
Blood Glucose
Leptin
Male
[SDV]Life Sciences [q-bio]
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Adipose tissue
MESH: Triiodothyronine
MESH: Recombinant Proteins
0302 clinical medicine
Endocrinology
Food Animals
MESH: Reverse Transcriptase Polymerase Chain Reaction
Gene expression
Insulin
MESH: Animals
ComputingMilieux_MISCELLANEOUS
MESH: Receptors
Cell Surface
MESH: Lipid Metabolism
2. Zero hunger
Sterol Regulatory Element Binding Proteins
0303 health sciences
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
Reverse Transcriptase Polymerase Chain Reaction
MESH: Gene Expression Regulation
Enzymologic
digestive
oral
and skin physiology
MESH: Chickens
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Fasting
MESH: Nutritional Status
Recombinant Proteins
[SDV] Life Sciences [q-bio]
Fatty acid synthase
Liver
Lipogenesis
Receptors
Leptin
Triiodothyronine
hormones
hormone substitutes
and hormone antagonists
MESH: Fatty Acid Synthases
MESH: Triglycerides
medicine.medical_specialty
MESH: Fasting
Nutritional Status
030209 endocrinology & metabolism
MESH: Sterol Regulatory Element Binding Proteins
Receptors
Cell Surface
MESH: Receptors
Leptin
MESH: Insulin
Biology
[INFO] Computer Science [cs]
Gene Expression Regulation
Enzymologic
03 medical and health sciences
LIPOGENESIS
Internal medicine
medicine
MESH: Blotting
Northern
Animals
[INFO]Computer Science [cs]
RNA
Messenger
Triglycerides
030304 developmental biology
MESH: RNA
Messenger
Leptin receptor
Lipid metabolism
MESH: Thyroxine
MESH: Leptin
medicine.disease
Blotting
Northern
Lipid Metabolism
Obesity
MESH: Male
Thyroxine
biology.protein
MESH: Blood Glucose
Animal Science and Zoology
Fatty Acid Synthases
Chickens
MESH: Liver
Zdroj: Domestic Animal Endocrinology
Domestic Animal Endocrinology, Elsevier, 2005, 29 (4), pp.646-660. ⟨10.1016/j.domaniend.2005.05.002⟩
Domestic Animal Endocrinology, Elsevier, 2005, 29, pp.646-660
ISSN: 0739-7240
DOI: 10.1016/j.domaniend.2005.05.002⟩
Popis: International audience; Leptin is reported to have direct effects on lipogenesis in peripheral tissues that are independent of its central effects on food intake and body weight. These experiments have been mainly carried out in rodents (different models of obesity) in which lipogenesis occurs in both adipose tissue and liver. Such effects are unknown in birds in which lipogenesis occurs essentially in the liver. In this study, leptin gene expression and circulating leptin levels were examined in two chicken lines, selected for high (FL) or low (LL) abdominal fat pad size, at different nutritional states (fasted and fed state). In addition, effects of recombinant chicken leptin on liver metabolism were investigated. Hepatic leptin and fatty acid synthase (FAS) gene expression and plasma leptin levels were significantly higher in FL than in LL chickens (P < 0.05). In both lines, fasting significantly reduced hepatic leptin and FAS mRNA levels (P < 0.05). Continuous administration of recombinant chicken leptin (8 microg/kg/h) during 6 h significantly inhibited food intake (51%) and increased leptinemia (23-fold) compared to untreated group. Despite the decrease of food intake, leptin significantly induced the expression of FAS in chicken liver. These changes were accompanied by a significant down-regulation of leptin receptor gene expression, however SREBP-1, the main transcription factor of lipogenic genes, remained unchanged. This result suggests a local potential role of leptin in the regulation of avian hepatic lipogenesis, and explain, at least partly, the metabolic changes evolved during the divergent selection of FL and LL chickens.
Databáze: OpenAIRE
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