Luminescent Silica Nanobeads: Characterization and Evaluation as Efficient Cytoplasmatic Transporters for T-Lymphocytes
| Autor: | Nicola Rosato, Tomas Mustelin, Massimo Bottini, Fabio Cerignoli, David M. Mills, Marilisa Leone, Maurizio Pellecchia, Federica D’Annibale, A Bergamaschi, Andrea Magrini |
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| Rok vydání: | 2007 |
| Předmět: |
CD28
Cytoplasm Luminescence CD3 Complex T-Lymphocytes CD3 Biotin Endocytosis Biochemistry Jurkat cells Article Antibodies Catalysis Cell Line chemistry.chemical_compound Colloid and Surface Chemistry CD28 Antigens Cell Line Tumor Humans Antigens Settore BIO/10 Cytotoxicity Tumor biology Chemistry Biological Transport General Chemistry Avidin Silicon Dioxide Nanoparticles Antigens CD3 Antigens CD28 biology.protein Luminophore Intracellular |
| Zdroj: | Journal of the American Chemical Society. 129:7814-7823 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja070245c |
| Popis: | We report the fabrication and characterization of neutravidin-conjugated silica nanobeads doped with a ruthenium-complex luminophore and functionalized with anti-human CD3 and anti-human CD28, and an acid-sensitive polymer. We observed that the nanobeads were readily delivered into Jurkat T leukemia cells by endocytosis, transported into lysosomes, and subsequently into the cytoplasm as revealed by pH-sensitive luminescence. Since signs of cytotoxicity were not observed, the reported nanobeads could be an excellent and non-toxic building block for efficient intracellular transporters. |
| Databáze: | OpenAIRE |
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