Poliovirus surveillance by examining sewage specimens. Quantitative recovery of virus after introduction into sewerage at remote upstream location
| Autor: | M. Stenvik, H. Partanen, A. Kangas, T. Hovi |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Epidemiology
viruses Population Sewage Biology medicine.disease_cause Poliovirus Type 1 Virus Cell Line Mice Sewerage medicine Animals Humans Serotyping education Finland education.field_of_study business.industry Poliovirus Virology Enterovirus B Human Infectious Diseases Enterovirus Separation method business Research Article Environmental Monitoring |
| Zdroj: | Epidemiology and Infection. 127 |
| ISSN: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268801005787 |
| Popis: | In order to assess the feasibility of environmental poliovirus surveillance, known amounts of poliovirus type 1, strain Sabin, were flushed into the sewage network of Helsinki. Grab specimens collected at a remote downstream location and concentrated about a 100-fold revealed infectious poliovirus on four successive days in all three separate experiments. As for concentration, a simple two-phase separation method was found to be at least as useful as a several-fold more resource-demanding polyethylene glycol (PEG) precipitation method. Recovery of the introduced virus was remarkably high (more than 10%). Using the current system, it might be possible to detect poliovirus circulation in a population of 700,000 people by examining a single 400 ml sewage specimen, if 1 out of 10,000 inhabitants were excreting the virus. It is concluded that environmental surveillance is a sensitive approach to monitor silent poliovirus circulation in populations served by a sewage network. |
| Databáze: | OpenAIRE |
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