The role of the cowpox virus crmA gene during intratracheal and intradermal infection of C57BL/6 mice
| Autor: | Lyle L. Moldawer, Richard W. Moyer, Amy L. MacNeill |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Gene Expression Regulation
Viral Intratracheal inoculation Cowpox viruses Intradermal inoculation Attenuation of cowpox virus Virulence Pathogenesis Biology Virulence factor Virus Microbiology 03 medical and health sciences Mice Viral Proteins Immune system Species Specificity Mouse model of smallpox Virology medicine Animals Humans Cowpox virus Serpins 030304 developmental biology 0303 health sciences 030306 microbiology Pneumonia medicine.disease crmA 3. Good health Mice Inbred C57BL Viral replication Caspases Poxvirus Smallpox |
| Zdroj: | Virology. 384(1):151-160 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2008.10.041 |
| Popis: | Intratracheal (i.t.) infection of mice with cowpox virus (CPXV), is lethal at a lower dose than intranasal (i.n.) inoculation. CPXV deleted for cytokine response modifier A (CPXVΔcrmA) was attenuated compared to CPXV after i.t. inoculation. This attenuation could not be attributed to differences in virus replication, immunomodulators, or cells infiltrating the lungs. Deletion of crmA also caused attenuation during intradermal (i.d.) infection. In contrast to i.t.-inoculated virus, deletion of crmA reduced virus replication at the site of infection. This difference correlated to increased numbers of CD3 + cells in CPXVΔcrmA-associated dermal lesions. Thus, crmA is a virulence factor in mice during either pulmonary or dermal cowpox infection; however the influence of crmA is more evident during i.d. inoculation. This suggests that the host immune response differs in the two routes of infection and emphasizes the need to consider the effect of route of infection when examining functions of virulence factors in vivo . |
| Databáze: | OpenAIRE |
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