Large-volume leukapheresis procedure for peripheral blood progenitor cell collection in children weighing 15 kg or less: Efficacy and safety evaluation
| Autor: | François Demeocq, Jean Bernard Palcoux, Jean Louis Stéphan, Jean-Pierre Vannier, Marc G. Berger, Lionel deLumley, Justyna Kanold, Céline Rouzier, Chantal Rapatel, Pascale Halle |
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| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
medicine.medical_specialty Erythrocytes Time Factors Anemia medicine.medical_treatment CD34 Antigens CD34 Hypothermia Hemoglobins Risk Factors Catheterization Peripheral Granulocyte Colony-Stimulating Factor medicine Humans Citrates Leukapheresis Progenitor cell Large volume leukapheresis Child Chemotherapy Blood Volume Platelet Count business.industry Body Weight Hematopoietic Stem Cell Transplantation Infant medicine.disease Thrombocytopenia Hematopoietic Stem Cell Mobilization Peripheral blood Clone Cells Surgery Apheresis Oncology Child Preschool Anesthesia Pediatrics Perinatology and Child Health Hypotension Safety business |
| Zdroj: | Medical and Pediatric Oncology. 32:7-10 |
| ISSN: | 1096-911X 0098-1532 |
| Popis: | Background We update our experience on large-volume leukapheresis (LVL) in very small patients with malignancies. LVLs were performed with the aim of reducing the psychological impact of leukaphereses by reducing the number of procedures while collecting large numbers of cells. Procedure Seventeen LVLs were performed using a Cobe Spectra separator in 14 patients weighing ⩽15 kg. A median of 3.8 patient's blood volumes corresponding to 296 mL/kg (range, 202–565) of blood was processed per session of 190 minutes (120–279) duration. A femoral catheter was installed specially for collection for 88% LVL (vs. 35% for standard leukaphereses). A median volume of 16.9 mL/kg was collected with 5.4 × 108 MNC/kg (range, 0.6–16.3) and 8.2 × 106 CD34+ cells/kg (range, 1.3–31.7). Results No signs of complications due to citrate toxicity were encountered. No hypotensive or hypothermic episodes were observed. Platelet counts were significantly diminished after each procedure (median: −59%). When the extracorporal line was not primed with red blood cells (RBC), the difference between pre-LVL and post-LVL hemoglobin levels was significant with a median 32 g/L decrease. Conclusions The LVL approach for peripheral blood progenitor cells (PBPC) collection in very small children may expose them to the risk of anemia and thrombocytopenia and an excess of special central line installation. The application of this technique in these patients should be reserved for special cases when a very large number of cells must be collected and should be performed by an experienced team. Med. Pediatr. Oncol. 32:7–10, 1999. © 1999 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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