A 300 μm Organotypic Bone Slice Culture Model for Temporal Investigation of Endochondral Osteogenesis
| Autor: | Paola Castrogiovanni, Tamilselvan Mohan, Ulrike Zefferer, Ehsan Bonyadi Rad, Giuseppe Musumeci, Annelie Weinberg, Sepideh Mostofi, Nicole G. Grün, Ute Schäfer, Markus Absenger-Novak, Sriveena Srinivasaiah |
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| Rok vydání: | 2019 |
| Předmět: |
Time Factors
Culture model 0206 medical engineering Biomedical Engineering Medicine (miscellaneous) Bioengineering 02 engineering and technology Biology bone slice model bone Rats Sprague-Dawley Tissue Culture Techniques 03 medical and health sciences Calcification Physiologic Osteogenesis medicine Animals Femur organotypic bone culture cartilage endochondral ossification bone morphogenesis Endochondral ossification Immature Bone 030304 developmental biology Bone morphogenesis 0303 health sciences Tissue Engineering Cartilage 020601 biomedical engineering Extracellular Matrix Cell biology medicine.anatomical_structure Calcium Bone Remodeling Crystallization Chondrogenesis Biomarkers Explant culture |
| Zdroj: | Tissue Engineering Part C: Methods. 25:197-212 |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2018.0368 |
| Popis: | Translational studies to elucidate the response of immature bone to biologic and physical stimuli have been held back by the lack of a viable long-term functional bone explant model. This study attempts to bridge this gap between cell culture and animal model studies. In this study, we describe a methodology to derive a 300 μm organotypic femur slice comprising physiological zones (epiphysis and meta-diaphysis) essential for endochondral bone development. The unique capability of slice culture model incorporating enhanced nutrient access to distinct bone tissue components associated with linear bone growth facilitates the investigation of the orchestrated cellular transition of chondrogenic and osteogenic cells involved in endochondral bone development in an |
| Databáze: | OpenAIRE |
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