Effects of Toll-like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis
| Autor: | Armstrong Murira, Leonard M. Millis, Jacqueline Vapenik, Lynette B. Sigola, Ana-Lucia Fuentes |
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| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Phagocytosis Saccharomyces cerevisiae Biology Ligands Cell morphology Microbiology Lipopeptides Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animals Macrophage Scavenger receptor Toll-Like Receptors Zymosan Cell Biology General Medicine TLR2 RAW 264.7 Cells 030104 developmental biology chemistry TLR5 TLR4 Flagellin 030215 immunology Developmental Biology |
| Zdroj: | Tissue and Cell. 48:389-396 |
| ISSN: | 0040-8166 |
| DOI: | 10.1016/j.tice.2016.04.002 |
| Popis: | In this study we compared the effects of the Toll-like receptor (TLR) ligands lipopolysaccharide (LPS), flagellin, the synthetic bacterial triacylated lipopeptide Pam3-Cys-Ser-Lys4 (Pam3CSK4), Polyinosinic:polycytidylic acid (Poly I:C), and macrophage-activating lipopeptide (MALP-2), which are TLR4, TLR5, TLR1/2, TLR3, and TLR2/6 agonists, respectively, on cell morphology and phagocytosis of zymosan particles, derived from Saccharomyces cerevisiae, and rich in fungal PAMPs including beta-glucan, mannose, and chitin. LPS, Pam3CSK4, and MALP-2 induced an activated macrophage phenotype and enhanced zymosan phagocytosis. In contrast, flagellin and Poly I:C, respectively, had little effect on cell morphology and phagocytosis. We examined the role of scavenger receptor A (SR-A) on zymosan phagocytosis. Cells cultured in medium alone expressed SR-A, and LPS induced further expression of the receptor. We also observed inhibitory effects of scavenger receptor antagonists fucoidan, dextran sulphate, and Polyinosinic (Poly I), respectively, on zymosan phagocytosis of cells in medium alone and those pre-treated with LPS. We conclude that exposure to specific TLR ligands impacts both cellular morphology and phagocytic capacity, and that scavenger receptors contribute to zymosan ingestion as well as LPS-induced augmentation of phagocytosis. |
| Databáze: | OpenAIRE |
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