Failure of intrathymic inoculation of donor-specific splenocytes to prolong cardiac or renal allograft survival in dogs
| Autor: | David K. C. Cooper, M. Niekrasz, H. Merhav, S Kosanke, Takaaki Kobayashi, Yigang Luo, D. Smith, Judy Baker, Sherian Xu Li, Y. Ye |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Graft Rejection
medicine.medical_specialty Globulin Cyclophosphamide medicine.medical_treatment Immunology Thymus Gland Gastroenterology Organ transplantation Lymphocyte Depletion Dogs Prednisone Internal medicine medicine Splenocyte Immunology and Allergy Animals Saline Antilymphocyte Serum Transplantation Kidney biology business.industry Graft Survival Immunosuppression Kidney Transplantation medicine.anatomical_structure biology.protein Heart Transplantation business Spleen medicine.drug |
| Zdroj: | Transplant immunology. 3(1) |
| ISSN: | 0966-3274 |
| Popis: | The intrathymic inoculation (ITI) of donor splenocytes into potential organ transplant recipients has been demonstrated to result in donor-specific unresponsiveness and greatly prolonged survival of subsequent organ allografts in rodents without the need for long-term pharmacological immunosuppressive therapy. We have studied the effect of the ITI of saline (controls) (groups 1 ( n = 6) and 3 ( n = 6)) or donor splenocytes (groups 2 ( n = 10) and 4 ( n = 8)) in dogs that received either pharmacological immunosuppression (with cyclosporine and prednisone, ±azathioprine/cyclophosphamide) (groups 1 and 2) or rabbit anti-dog antithymocyte globulin (groups 3 and 4) at the time of ITI. Kidney or heart allografting (from the donor of the splenocytes) was carried out 16–74 days after ITI; all but four transplants were performed within 16–22 days after ITI. Mean kidney allograft survival was 6, 10, 9, and 9 days, respectively, in groups 1–4. Mean cardiac allograft survival was 7, 14, 8, and 7 days, respectively. There was no statistical difference in allograft survival between those dogs that received ITI of saline and those that received donor splenocytes. These results would suggest that the protocols developed to date using ITI in rodent species may not be successful in dogs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect