The fibrinogen cleavage product A -Val360, a specific marker of neutrophil elastase activity in vivo
| Autor: | Qian Si, Richard I Carter, Ali Ahmad, Richard A. Mumford, Asger Dirksen, Kelly M. Treonze, Robert A. Stockley, Paul E. Finke, P. Davies, John L. Humes, Eeva Piitulainen |
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| Rok vydání: | 2011 |
| Předmět: |
Pulmonary and Respiratory Medicine
Exacerbation Pilot Projects Placebo Fibrinogen Neutrophil Activation Pathogenesis Double-Blind Method alpha 1-Antitrypsin Deficiency Humans Medicine Calcimycin Peroxidase COPD Alpha 1-antitrypsin deficiency Dose-Response Relationship Drug biology business.industry medicine.disease Peptide Fragments Pulmonary Emphysema alpha 1-Antitrypsin Neutrophil elastase Immunology biology.protein Sputum medicine.symptom Leukocyte Elastase business Leukocyte L1 Antigen Complex Biomarkers medicine.drug |
| Zdroj: | Thorax. 66:686-691 |
| ISSN: | 0040-6376 |
| Popis: | Background Alpha-1-antitrypsin (A1AT) deficiency is the only recognised genetic risk factor for chronic obstructive pulmonary disease (COPD), a leading cause of morbidity and mortality worldwide. Since A1AT is the major inhibitor of neutrophil elastase (NE), this enzyme has become widely implicated in the pathogenesis of COPD in general; however, there is currently no specific biomarker for its pre-inhibition activity. Such a biomarker should be a measure of elastase-specific COPD disease activity with the potential to assess early targeted therapeutic intervention, in contrast to traditional and non-specific disease severity markers such as forced expiratory volume in 1 s. Methods In pilot studies, plasma Aα-Val 360 and markers of neutrophil activation were measured in 95 subjects with a range of A1AT concentrations. Aα-Val 360 and sputum elastase activity were also measured in a further seven PiZ A1AT-deficient subjects over the course of an acute exacerbation. Finally, Aα-Val 360 was measured in plasma from subjects randomised to receive A1AT replacement or placebo in the EXACTLE trial. Results The plasma concentrations of Aα-Val 360 and A1AT related exponentially, consistent with previous theoretical and in vitro experimental data. L-233 (an intracellular NE inhibitor) blocked generation of Aα-Val 360 and subsequent A1AT/NE complex formation. Aα-Val 360 was related to the spirometric severity of lung disease in A1AT deficiency, to sputum elastase activity in acute exacerbations and was decreased in subjects receiving A1AT replacement therapy (while remaining constant in those receiving placebo). Conclusions Aα-Val 360 represents the first specific footprint of pre-inhibition NE activity and is a potential biomarker of disease activity and progression in subjects with elastase-dependent COPD. Trial registration The EXACTLE study was registered in ClinicalTrials.gov as ‘Antitrypsin (AAT) to Treat Emphysema in AAT-Deficient Patients’; ClinicalTrials.gov Identifier: NCT00263887. |
| Databáze: | OpenAIRE |
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