Polysomes and Protein Synthesis in Cells Infected with a DNA Virus
| Autor: | Robert J. Sydiskis, Bernard Roizman |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
chemistry.chemical_classification
Carbon Isotopes Virus Cultivation Multidisciplinary Stimulation In Vitro Techniques Biology medicine.disease_cause Ribosome Molecular biology Virus Amino acid Viral Proteins Herpes simplex virus chemistry Cytoplasm Protein Biosynthesis Polysome medicine Protein biosynthesis Simplexvirus Radiometry Ribosomes |
| Zdroj: | Science. 153:76-78 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.153.3731.76 |
| Popis: | In HEp-2 cells infected with herpes simplex virus the rate of protein synthesis at first decline, is stimulated between 4 and 8 hours after infection, and progressively and irreversibly declines from 9 to 16 hours later. The increase and decrease in rates coincide with corresponding changes in the amounts of cytoplasmic polysomes and amounts of labeled amino acids in nascent peptides bound to polysomes. The data indicate that (i) early and late inhibition and intervening stimulation of protein synthesis are due to the corresponding breakdown and formation of polysomes, and (ii) the bulk of viral proteins is probably made on cytoplasmic polysomes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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