Determination of khellin and visnagin in Ammi visnaga fruits by capillary electrophoresis
| Autor: | F.B. Erim, Keriman Günaydin |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Detection limit
Ammi Khellin Chromatography biology Chemistry Organic Chemistry Electrophoresis Capillary General Medicine biology.organism_classification Biochemistry Sensitivity and Specificity Micellar electrokinetic chromatography Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Furanochromone Ammi visnaga Visnagin Quantitative analysis (chemistry) |
| Zdroj: | Journal of chromatography. A. 954(1-2) |
| ISSN: | 0021-9673 |
| Popis: | A new, simple and rapid capillary electrophoresis method was developed for the identification and quantitative determination of two medically active constituents-khellin and visnagin-in the extracts of Ammi visnaga fruits. Micellar electrochromatographic separation of khellin and visnagin was carried out using 10 mmol/l borate, 50 mmol/l sodium dodecylsulfate, 25% (v/v) acetonitrile at pH 9 as running buffer. Ammi visnaga fruits were extracted with methanol and the extracts were directly injected without any purification and pre-separation processes. Coumarin was used as internal standard for quantitation and the limits of detection for khellin and visnagin were 2.36 and 1.97 microg/ml, respectively using UV detection at 245 nm. |
| Databáze: | OpenAIRE |
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