Identification of genes with allelic imbalance on 6p associated with nasopharyngeal carcinoma in southern Chinese
| Autor: | Yan Li, Li Fu, Alissa Michelle Go Wong, Yan-Hui Fan, Miao-Xin Li, Jin-Xin Bei, Wei-Hua Jia, Yi-Xin Zeng, Danny Chan, Kenneth M C Cheung, Pak Sham, Daniel Chua, Xin-Yuan Guan, You-Qiang Song |
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| Rok vydání: | 2010 |
| Předmět: |
China
China - epidemiology Science Nasopharyngeal neoplasm Single-nucleotide polymorphism Human leukocyte antigen Biology Allelic Imbalance Polymorphism Single Nucleotide law.invention law medicine Humans Genetic Predisposition to Disease GABBR1 Genetics and Genomics/Cancer Genetics Polymerase chain reaction Adaptor Proteins Signal Transducing Nasopharyngeal Neoplasms - genetics Genetics Multidisciplinary Oncology/Head and Neck Cancers Haplotype Nasopharyngeal Neoplasms Genetics and Genomics/Gene Expression medicine.disease Phosphoproteins Molecular biology Gene Expression Regulation Neoplastic Nasopharyngeal carcinoma Receptors GABA-B Case-Control Studies Medicine Chromosomes Human Pair 6 Chromosomes Human Pair 6 - genetics Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 1, p e14562 (2011) |
| ISSN: | 1932-6203 |
| Popis: | Nasopharyngeal carcinoma (NPC) is a malignancy of epithelial origin. The etiology of NPC is complex and includes multiple genetic and environmental factors. We employed case-control analysis to study the association of chromosome 6p regions with NPC. In total, 360 subjects and 360 healthy controls were included, and 233 single nucleotide polymorphisms (SNPs) on 6p were examined. Significant single-marker associations were found for SNPs rs2267633 (p = 4.49610 -5), rs2076483 (most significant, p = 3.36610 -5), and rs29230 (p = 1.43610 -4). The highly associated genes were the gamma-amino butyric acid B receptor 1 (GABBR1), human leukocyte antigen (HLA-A), and HLA complex group 9 (HCG9). Haplotypic associations were found for haplotypes AAA (located within GABBR1, p-value = 6.46610 -5) and TT (located within HLA-A, p = 0.0014). Further investigation of the homozygous genotype frequencies between cases and controls suggested that micro-deletion regions occur in GABBR1 and neural precursor cell expressed developmentally down-regulated 9 (NEDD9). Quantitative real-time polymerase chain reaction (qPCR) using 11 pairs of NPC biopsy samples confirmed the significant decline in GABBR1 and NEDD9 mRNA expression in the cancer tissues compared to the adjacent non-tumor tissue (p published_or_final_version |
| Databáze: | OpenAIRE |
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