Enhanced expression of decay-accelerating factor, a complement-regulatory protein, in the specialized intestinal metaplasia of Barrett's esophagus
| Autor: | Hiroaki Okazaki, Motowo Mizuno, Yasushi Shiratori, Chiho Makidono, Kazuhide Yamamoto, Junichirou Nasu, Hiroyuki Okada, Teizo Fujita, Sakiko Hiraoka, Ryo Terada |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Biology Pathology and Forensic Medicine Immunoenzyme Techniques Barrett Esophagus Esophagus medicine Gastric mucosa Humans RNA Messenger Intestinal Mucosa Fluorescent Antibody Technique Indirect Decay-accelerating factor Aged Aged 80 and over Metaplasia CD55 Antigens Esophageal disease Reverse Transcriptase Polymerase Chain Reaction fungi Intestinal metaplasia General Medicine Middle Aged medicine.disease Epithelium medicine.anatomical_structure Barrett's esophagus Adenocarcinoma Female Microdissection Biomarkers |
| Zdroj: | The Journal of laboratory and clinical medicine. 143(4) |
| ISSN: | 0022-2143 |
| Popis: | Intestinal-type epithelium in Barrett's esophagus, so-called specialized intestinal metaplasia (SIM), is a risk factor for the development of esophageal adenocarcinoma. Surface expression of decay-accelerating factor (DAF), a complement-regulatory protein, is markedly enhanced in intestinal metaplasia of the gastric mucosa. We therefore examined DAF expression in areas of SIM in Barrett's esophagus in an attempt to determine whether DAF is a biomarker of SIM. We obtained 53 endoscopic biopsy specimens from the esophageal columnar mucosae of 45 patients. We immunohistochemically examined the distribution of DAF and 2 other complement-regulatory proteins: homologous restriction factor-20 and membrane cofactor protein. We also examined the expression of DAF messenger RNA in SIM with the use of laser-capture microdissection and reverse transcription-polymerase chain reaction. Of the 53 specimens, 10 were found histologically to involve areas of SIM, 41 were SIM-negative epithelium, and 2 comprised areas of SIM and SIM-negative epithelium. DAF staining was negligible in 35 of 43 specimens of the SIM-negative columnar epithelium, but DAF was strongly stained on the apical surface in all 12 SIM-positive specimens (P |
| Databáze: | OpenAIRE |
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