Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
| Autor: | Matías Leonel Alegre, María Victoria Martin, Gabriela Carolina Pagnussat, Federico Berdun, Carlos Guillermo Bartoli, Charlotte Steelheart, Graciela L. Salerno, Anabella Aguillera |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cyanobacteria
Antioxidant Strategy and Management medicine.medical_treatment Biología Cell medicine.disease_cause Industrial and Manufacturing Engineering Antioxidants chemistry.chemical_compound medicine Methods Article Ciencias Naturales biology Mechanical Engineering Metals and Alloys Glutathione biology.organism_classification Ascorbic acid medicine.anatomical_structure Synechocystis sp Biochemistry chemistry Oxidative stress Second messenger system |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP Bio Protoc |
| Popis: | Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803. Instituto de Fisiología Vegetal |
| Databáze: | OpenAIRE |
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