P-body-induced inactivation of let-7a miRNP prevents the death of growth factor-deprived neuronal cells
| Autor: | Somi Patranabis, Suvendra N. Bhattacharyya |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Programmed cell death Cellular differentiation medicine.medical_treatment Apoptosis Cytoplasmic Granules Biochemistry PC12 Cells 03 medical and health sciences 0302 clinical medicine Gene expression Genetics medicine Animals Molecular Biology Neurons Chemistry Growth factor RNA Translation (biology) Argonaute Cell biology Rats MicroRNAs 030104 developmental biology Argonaute Proteins Intercellular Signaling Peptides and Proteins Ectopic expression 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 32(3) |
| ISSN: | 1530-6860 |
| Popis: | RNA processing bodies (P-bodies) are cytoplasmic RNA granules in eukaryotic cells that regulate gene expression by executing the translation suppression and degradation of mRNAs that are targeted to these bodies. P-bodies can also serve as storage sites for translationally repressed mRNAs both in mammalian cells and yeast cells. In this report, a unique role of mammalian P-bodies is documented. Depletion of P-body components dedifferentiate nerve growth factor-treated PC12 cells, whereas ectopic expression of P-body components induces the neuronal differentiation of precursor cells. Trophic factor withdrawal from differentiated cells induces a decrease in cellular P-body size and numbers that are coupled with dedifferentiation and cell death. Here, we report how the expression of P-body proteins-by ensuring the phosphorylation of argonaute protein 2 and the subsequent inactivation let-7a miRNPs-prevents the apoptotic death of growth factor-depleted neuronal cells.-Patranabis, S., Bhattacharyya, S. N. P-body-induced inactivation of let-7a miRNP prevents the death of growth factor-deprived neuronal cells. |
| Databáze: | OpenAIRE |
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