Performance of point of care assays for hepatitis B and C viruses in chronic kidney disease patients
| Autor: | Danielle Malta Lima, Jeová Keny Baima Colares, Juliana Custódio Miguel, Jakeline Ribeiro Barbosa, Livia Melo Villar, Moyra Machado Portilho, Vanessa Alves Marques, Elisabeth Lampe, Cristianne Sousa Bezerra, Natália Vasconcelos de Souza, Lia Laura Lewis-Ximenez, Vanessa Faria Cortes |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Hepatitis B virus medicine.medical_specialty HBsAg Adolescent Hepatitis C virus Hepacivirus Population medicine.disease_cause Sensitivity and Specificity Gastroenterology Pathology and Forensic Medicine Young Adult 03 medical and health sciences Hepatitis B Chronic 0302 clinical medicine Internal medicine medicine Humans 030212 general & internal medicine Renal Insufficiency Chronic education Aged Aged 80 and over Hepatitis education.field_of_study biology business.industry virus diseases General Medicine Hepatitis C Chronic Middle Aged Hepatitis B medicine.disease biology.organism_classification digestive system diseases Point-of-Care Testing Female 030211 gastroenterology & hepatology business Kidney disease |
| Zdroj: | Journal of Clinical Pathology. 71:879-884 |
| ISSN: | 1472-4146 0021-9746 |
| Popis: | AimsPoint of care testing (POCT) has been used for hepatitis B and C diagnosis in general population, but little is known about the influence of clinical conditions in the accuracy of these assays. This study aims to evaluate the performance of POCTs for detection of hepatitis B virus surface antigen (HBsAg) and antibodies to Hepatitis C Virus (anti-HCV) in Chronic Kidney Disease (CKD) patients.MethodsA total of 286 subjects were included in this study. HBsAg and anti-HCV were detected using commercial EIAs and four POCTs: HBsAg (WAMA Imuno-Rápido HBsAg and VIKIA HBsAg) and anti-HCV (DOLES HCV teste rápido and WAMA Imuno-Rápido anti-HCV) in serum and whole blood.ResultsUsing EIA, HBsAg and anti-HCV prevalence was 4.5% and 16.1% in CKD patients. HBsAg and anti-HCV POCTs had sensitivities from 92.3% to 100% and 84.8% to 89.1% while specificities were 99.3% to 100% and 99.2% to 99.6%, respectively. POCT using serum samples performed well compared with whole blood samples and true positive samples of POCTs had high optical density to cut-off (OD/CO) values compared with EIA.ConclusionsThis study demonstrates good performance of HBsAg and anti-HCV POCTs in CKD patients, especially in serum samples indicating low interference of this disease in the performance of these assays. POCTs could be an important tool for HBV and HCV screening in high-risk populations. |
| Databáze: | OpenAIRE |
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