Near-infrared triggered co-delivery of doxorubicin and quercetin by using gold nanocages with tetradecanol to maximize anti-tumor effects on MCF-7/ADR cells
| Autor: | Yanna Yu, Yun Wang, Shengtang Huang, Zhipeng Zhang, Yuanyuan Shen, Hao Zhu, Shengrong Guo, Fangzhou Li, Shaohui Xu |
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| Rok vydání: | 2018 |
| Předmět: |
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Cell Survival Biotin Metal Nanoparticles Antineoplastic Agents Apoptosis 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences Biomaterials Colloid and Surface Chemistry Nanocages Cell Line Tumor medicine Humans Doxorubicin ATP Binding Cassette Transporter Subfamily B Member 1 Particle Size Cytotoxicity Drug Carriers Liposome Chemistry Cell Cycle technology industry and agriculture Combination chemotherapy Phototherapy 021001 nanoscience & nanotechnology 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Multiple drug resistance Drug Liberation Drug Resistance Neoplasm Drug delivery Quercetin Gold Fatty Alcohols Nanocarriers Reactive Oxygen Species 0210 nano-technology medicine.drug |
| Zdroj: | Journal of Colloid and Interface Science. 509:47-57 |
| ISSN: | 0021-9797 |
| DOI: | 10.1016/j.jcis.2017.08.097 |
| Popis: | Previously, combination chemotherapy of doxorubicin (DOX) and quercetin (QUR) was developed to improve antitumor effects and reverse multidrug resistance and several biocompatible nanocarriers, such as liposomes and micelles, were validated for their targeted delivery. In this study, we report a near-infrared (NIR)-responsive drug delivery system based on DOX and QUR co-loaded gold nanocages (AuNCs) with biotin modification. The system was simply fabricated by filling the hollow interiors of AuNCs with tetradecanol (TD), a phase-change material with a melting point of 39°C, to control the drug release. The main cause of multidrug resistance (MDR) of DOX is the overexpression of P-glycoprotein (P-gp), which can be inhibited by QUR. Thus the combination chemotherapy of DOX and QUR may provide a promising strategy for MDR. The in vitro cytotoxicity of DOX and QUR at several fixed mass ratios was carried out and showed that the combination index (CI) was the smallest at the ratio of 1:0.2, indicating that the best synergistic effect was achieved. The resultant nanocomplex (abbreviated as BPQD-AuNCs) exhibited fast release (80% released in 20min) and strong cytotoxicity against MCF-7/ADR cells (IC50, 1.5μg/mL) under NIR irradiation. Additionally, BPQD-AuNCs were found to generate a large amount of reactive oxygen species (ROS), to inhibit P-gp expression and ATP activity. Taken together, the results show that BPQD-AuNC is a prospective nano-delivery system for overcoming multidrug-resistant cancer. |
| Databáze: | OpenAIRE |
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