Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model
| Autor: | Bryce Nelson, Hironori Bando, Sugao Oshiro, Shinya Fukumoto, Namal Perera, Hirotaka Kanuka, Naoaki Shinzawa, Hiroka Aonuma, Aya Yoshimura |
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| Rok vydání: | 2008 |
| Předmět: |
Veterinary medicine
biology Transmission (medicine) Research Loop-mediated isothermal amplification Dirofilaria immitis Aedes aegypti medicine.disease biology.organism_classification Virology Filariasis lcsh:Infectious and parasitic diseases Infectious Diseases Parasitology Vector (epidemiology) medicine lcsh:RC109-216 Arthropod Vector |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 2, Iss 1, p 15 (2009) |
| ISSN: | 1756-3305 |
| Popis: | Background Despite recent advances in our understanding of the basic biology behind transmission of zoonotic infectious diseases harbored by arthropod vectors these diseases remain threatening public health concerns. For effective control of vector and treatment, precise sampling indicating the prevalence of such diseases is essential. With an aim to develop a quick and simple method to survey zoonotic pathogen-transmitting vectors, LAMP (loop-mediated isothermal amplification) was applied to the detection of filarial parasites using a filarial parasite-transmitting experimental model that included one of the mosquito vectors, Aedes aegypti, and the canine heartworm, Dirofilaria immitis. Results LAMP reactions amplifying the cytochrome oxidase subunit I gene demonstrated high sensitivity when a single purified D. immitis microfilaria was detected. Importantly, the robustness of the LAMP reaction was revealed upon identification of an infected mosquito carrying just a single parasite, a level easily overlooked using conventional microscopic analysis. Furthermore, successful detection of D. immitis in wild-caught mosquitoes demonstrated its applicability to field surveys. Conclusion Due to its simplicity, sensitivity, and reliability, LAMP is suggested as an appropriate diagnostic method for routine diagnosis of mosquito vectors carrying filarial parasites. This method can be applied to the survey of not only canine filariasis but also lymphatic filariasis, another major public health problem. Therefore, this method offers great promise as a useful diagnostic method for filarial parasite detection in endemic filariasis regions. |
| Databáze: | OpenAIRE |
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