Second Era of OMICS in Caries Research: Moving Past the Phase of Disillusionment
| Autor: | J.M. Ten Cate, Marcelle M. Nascimento, Nobuhiro Takahashi, Alex Mira, E. Zaura |
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| Rok vydání: | 2017 |
| Předmět: |
Proteomics
0301 basic medicine Clinomics Dental Research Reviews microbiome Computational biology Dental Caries Biology Bioinformatics Genome biofilm 03 medical and health sciences 0302 clinical medicine medicine Metabolome Humans Metabolomics Microbiome genome General Dentistry Microbiota Computational Biology Genomics 030206 dentistry medicine.disease Omics 030104 developmental biology Biofilms Data quality dental caries Metagenome next-generation sequencing metabolome Lower cost Diffusion of Innovation Dysbiosis |
| Zdroj: | Nascimento, M M, Zaura, E, Mira, A, Takahashi, N & ten Cate, J M 2017, ' Second Era of OMICS in Caries Research: Moving Past the Phase of Disillusionment ', Journal of Dental Research, vol. 96, no. 7, pp. 733-740 . https://doi.org/10.1177/0022034517701902 JOURNAL OF DENTAL RESEARCH r-FISABIO. Repositorio Institucional de Producción Científica instname r-FISABIO: Repositorio Institucional de Producción Científica Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO) |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/0022034517701902 |
| Popis: | Novel approaches using OMICS techniques enable a collective assessment of multiple related biological units, including genes, gene expression, proteins, and metabolites. In the past decade, next-generation sequencing (NGS) technologies were improved by longer sequence reads and the development of genome databases and user-friendly pipelines for data analysis, all accessible at lower cost. This has generated an outburst of high-Throughput data. The application of OMICS has provided more depth to existing hypotheses as well as new insights in the etiology of dental caries. For example, the determination of complete bacterial microbiomes of oral samples rather than selected species, together with oral metatranscriptome and metabolome analyses, supports the viewpoint of dysbiosis of the supragingival biofilms. In addition, metabolome studies have been instrumental in disclosing the contributions of major pathways for central carbon and amino acid metabolisms to biofilm pH homeostasis. New, often noncultured, oral streptococci have been identified, and their phenotypic characterization has revealed candidates for probiotic therapy. Although findings from OMICS research have been greatly informative, problems related to study design, data quality, integration, and reproducibility still need to be addressed. Also, the emergence and continuous updates of these computationally demanding technologies require expertise in advanced bioinformatics for reliable interpretation of data. Despite the obstacles cited above, OMICS research is expected to encourage the discovery of novel caries biomarkers and the development of next-generation diagnostics and therapies for caries control. These observations apply equally to the study of other oral diseases. |
| Databáze: | OpenAIRE |
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