Morphological Changes Induced by Insulin-Like Growth Factor-I Gene Therapy in Pituitary Cell Populations in Experimental Prolactinomas
| Autor: | Claudia B. Hereñú, Gisela Camihort, Rodolfo G. Goya, Gloria M. Cónsole, Silvia Susana Rodríguez, María Bracamonte, Georgina Luna |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Pituitary gland
medicine.medical_specialty Histology Somatotropic cell medicine.drug_class medicine.medical_treatment Genetic Vectors Insulin-like growth factor type I Prolactinomas Biology Rats Sprague-Dawley Prolactin cell Insulin-like growth factor Gene therapy Internal medicine medicine Animals Prolactinoma Insulin-Like Growth Factor I Original Paper Pituitary cell populations Pituitary tumors Genetic Therapy medicine.disease Estrogen Prolactin Rats medicine.anatomical_structure Endocrinology Pituitary Gland Models Animal Ciencias Médicas Rat Female Corticotropic cell Anatomy |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| ISSN: | 1422-6421 1422-6405 |
| DOI: | 10.1159/000258701 |
| Popis: | In previous studies, we assessed the effects of intrapituitary injection of a recombinant adenoviral vector (RAd) harboring the cDNA for rat insulin-like growth factor type I (RAd-IGF-I) on the lactotrope and somatotrope populations in estrogen-induced prolactinomas. In the present study, we aimed to confirm these findings and further analyze the effect of transgenic RAd-IGF-I on the other pituitary cell populations in female rats. All animals except the intact group (no estrogen and no stereotaxic injection) received subcutaneous estrogen for 30 days, and the groups which received RAd-IGF-I or RAd expressing green fluorescent protein (control) were additionally treated with the appropriate vectors on experimental day 0. The RAd-IGF-I group showed a significant decrease in serum growth hormone and prolactin levels and lactotrope and somatotrope cell size induced by estrogen treatment. Cell density was not affected by 7 days of IGF-I gene therapy. Estrogen had an inhibitory effect on thyrotrope cell density, whereas with RAd-IGF-I there was a nonsignificant trend towards restoration of cell density, without changes in cell size. RAd-IGF-I treatment decreased corticotrope cell size without changing cell density. Estrogen decreased gonadotrope cell size and density, which was reversed by RAd-IGF-I. We conclude that in estrogen-induced pituitary tumors, IGF-I gene therapy has inhibitory effects on the lactotrope, somatotrope and corticotrope populations, while reversing the effect of estrogen on gonadotropic cells. Facultad de Ciencias Médicas Instituto de Investigaciones Bioquímicas de La Plata |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|