Identification of sperm proteins as candidate biomarkers for the analysis of reproductive isolation in Mytilus: a case study for the enkurin locus

Autor:	Humberto Quesada, Kai Postel, Stefanie Hartmann, Karola Lehmann, Katja Heilmann, Heiko Stuckas, Manuela Bartel, Eva Philipp, Burkhard Micheel
Rok vydání:	2012
Předmět:	Genetics Species complex Ecology biology Locus (genetics) Reproductive isolation Aquatic Science biology.organism_classification Sperm Mytilus Allele Gene Institut für Biochemie und Biologie Ecology Evolution Behavior and Systematics Sperm motility
Zdroj:	Marine Biology. 159:2195-2207
ISSN:	1432-1793 0025-3162
DOI:	10.1007/s00227-012-2005-7
Popis:	Sperm proteins of the marine sessile mussels of the Mytilus edulis species complex are models to investigate reproductive isolation and speciation. This study aimed at identifying sperm proteins and their corresponding genes. This was aided by the use of monoclonal antibodies that preferentially bind to yet unknown sperm molecules. By identifying their target molecules, this approach identified proteins with relevance to Mytilus sperm function. This procedure identified 16 proteins, for example, enkurin, laminin, porin and heat shock proteins. The potential use of these proteins as genetic markers to study reproductive isolation is exemplified by analysing the enkurin locus. Enkurin evolution is driven by purifying selection, the locus displays high levels of intraspecific variation and species-specific alleles group in distinct phylogenetic clusters. These findings characterize enkurin as informative candidate biomarker for analyses of clinal variation and differential introgression in hybrid zones, for example, to understand determinants of reproductive isolation in Baltic Mytilus populations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b21c03988c883392e848966664fe57f https://doi.org/10.1007/s00227-012-2005-7 Zobrazit plný text záznamu Plný text ve formátu PDF