Evaluation of an alkaline phosphatase-labeled oligonucleotide probe for the detection and enumeration of the thermostable-related hemolysin (trh) gene of Vibrio parahaemolyticus
| Autor: | Rachel Rangdale, Andrea M. B. Phillips, Shelley L. Murray, Jessica L. Nordstrom, Angelo DePaola, Michael C. L. Vickery, Sariqa Wagley |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
endocrine system
Virulence Food Contamination Biology Microbiology Hemolysin Proteins Bacterial Proteins Species Specificity Vibrionaceae Enumeration Animals Humans Vibrio parahaemolyticus food and beverages Hemolysin biochemical phenomena metabolism and nutrition equipment and supplies biology.organism_classification Alkaline Phosphatase United Kingdom United States Seafood Consumer Product Safety Food Microbiology bacteria Alkaline phosphatase Oligomer restriction Oligonucleotide Probes Water Microbiology Bacteria Food Science |
| Zdroj: | Journal of food protection. 69(11) |
| ISSN: | 0362-028X |
| Popis: | Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|