TMF/ARA160 Governs the Dynamic Spatial Orientation of the Golgi Apparatus during Sperm Development
| Autor: | Tali Lerer-Goldstein, Uri Nir, Tatiana Babushkin, Shai Bel, Smadar Levin-Zaidman, Roni Rahimi, Sally Shpungin, Yoav Elkis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Spermiogenesis Ubiquitin-Protein Ligases Vesicular Transport Proteins Golgi Apparatus lcsh:Medicine Microtubules symbols.namesake Mice Microtubule Sequence Analysis Protein Tubulin medicine Animals Nuclear membrane Acrosome Spermatogenesis lcsh:Science Mice Inbred ICR Multidisciplinary Spermatid biology Chemistry lcsh:R Golgi Matrix Proteins Cell Differentiation Golgi apparatus Spermatids Cell biology Protein Structure Tertiary DNA-Binding Proteins medicine.anatomical_structure symbols biology.protein NIH 3T3 Cells Chromatoid body lcsh:Q Gene Deletion Transcription Factors Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 12, p e0145277 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | TMF/ARA160 is known to be a TATA element Modulatory Factor (TMF). It was initially identified as a DNA-binding factor and a coactivator of the Androgen receptor. It was also characterized as a Golgi-associated protein, which is essential for acrosome formation during functional sperm development. However, the molecular roles of TMF in this intricate process have not been revealed. Here, we show that during spermiogenesis, TMF undergoes a dynamic change of localization throughout the Golgi apparatus. Specifically, TMF translocates from the cis-Golgi to the trans-Golgi network and to the emerging vesicles surface, as the round spermatids develop. Notably, lack of TMF led to an abnormal spatial orientation of the Golgi and to the deviation of the trans-Golgi surface away from the nucleus of the developing round spermatids. Concomitantly, pro-acrosomal vesicles derived from the TMF-/- Golgi lacked targeting properties and did not tether to the spermatid nuclear membrane thereby failing to form the acrosome anchoring scaffold, the acroplaxome, around the cell-nucleus. Absence of TMF also perturbed the positioning of microtubules, which normally lie in proximity to the Golgi and are important for maintaining Golgi spatial orientation and dynamics and for chromatoid body formation, which is impaired in TMF-/- spermatids. In-silico evaluation combined with molecular and electron microscopic analyses revealed the presence of a microtubule interacting domain (MIT) in TMF, and confirmed the association of TMF with microtubules in spermatogenic cells. Furthermore, the MIT domain in TMF, along with microtubules integrity, are required for stable association of TMF with the Golgi apparatus. Collectively, we show here for the first time that a Golgi and microtubules associated protein is crucial for maintaining proper Golgi orientation during a cell developmental process. |
| Databáze: | OpenAIRE |
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