ISME Journal

Autor: Long Tian, Christopher T. Lefèvre, Austin Reed, Sophie LeBlanc, Haijie Liu, Caroline L. Monteil, Boris A. Vinatzer, Parul Sharma, Noam Eckshtain-Levi, Shu Yang, Nicolas Menguy, Kevin Failor, Marco E. Mechan Llontop, Liangcheng Du
Přispěvatelé: Virginia Tech [Blacksburg], Michigan State University System, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Institut de recherche pour le développement [IRD] : UR206-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Nebraska–Lincoln, University of Nebraska System, Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), University of Nebraska [Lincoln]
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: ISME Journal
ISME Journal, inPress, ⟨10.1038/s41396-021-01140-4⟩
ISME Journal, Nature Publishing Group, In press, ⟨10.1038/s41396-021-01140-4⟩
ISME Journal, In press, ⟨10.1038/s41396-021-01140-4⟩
ISSN: 1751-7362
1751-7370
Popis: Earth's radiation budget and frequency and intensity of precipitation are influenced by aerosols with ice nucleation activity (INA), i.e., particles that catalyze the formation of ice. Some bacteria, fungi, and pollen are among the most efficient ice nucleators but the molecular basis of INA is poorly understood in most of them. Lysinibacillus parviboronicapiens (Lp) was previously identified as the first Gram-positive bacterium with INA. INA of Lp is associated with a secreted, nanometer-sized, non-proteinaceous macromolecule or particle. Here a combination of comparative genomics, transcriptomics, and a mutant screen showed that INA in Lp depends on a type I iterative polyketide synthase and a non-ribosomal peptide synthetase (PKS-NRPS). Differential filtration in combination with gradient ultracentrifugation revealed that the product of the PKS-NRPS is associated with secreted particles of a density typical of extracellular vesicles and electron microscopy showed that these particles consist in "pearl chain"-like structures not resembling any other known bacterial structures. These findings expand our knowledge of biological INA, may be a model for INA in other organisms for which the molecular basis of INA is unknown, and present another step towards unraveling the role of microbes in atmospheric processes. National Science FoundationNational Science Foundation (NSF) [IOS-1754721]; Virginia Agricultural Experiment Station; Hatch Program of the National Institute of Food and Agriculture, US Department of Agriculture Published version This study was supported by the National Science Foundation (IOS-1754721). Funding to Boris A. Vinatzer was also provided in part by the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, US Department of Agriculture. We would like to thank Kate Costello, Nakysa Kheirandish, Michelle Zambrano, and Nnanna Onyekaba for help with the droplet freezing assays.
Databáze: OpenAIRE
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