[1,2,3,4-13C] testosterone and [1,2,3,4-13C] estradiol
| Autor: | Hans Wynberg, Nick M. Drayer, Gijsbert Zomer |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
medicine.medical_specialty
Chemical Phenomena Stereochemistry medicine.drug_class medicine.medical_treatment Clinical Biochemistry Biochemistry Steroid chemistry.chemical_compound Endocrinology In vivo Internal medicine medicine Testosterone Molecular Biology Pharmacology Carbon Isotopes Estradiol Chemistry Organic Chemistry Acetal Aromatization Total synthesis Androgen Estrogen |
| Zdroj: | Steroids. 44(4) |
| ISSN: | 0039-128X |
| Popis: | The preparation of [1,2,3,4-13C] testosterone and of [1,2,3,4-13C] estradiol by total synthesis is described. The 13C labels are introduced by alkylating intermediate 1 with [1,2,3,4-13C]l-iodo-3,3-ethylenedioxybutane ( 2 ) to obtain intermediate 10 . Hydrolysis of the ketal function, cyclization, aromatization and removal of protective groups gave [1,2,3,4-13C] estradiol. Labeled testosterone was prepared by methylating intermediate 10 and by subsequent treatment with acid. The labeled steroids can be used as tracers for in vivo metabolic studies and as internal standards for the development of definitive gc-ms quantitative methods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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