Dual-Porosity Hollow Nanoparticles for the Immunoprotection and Delivery of Nonhuman Enzymes
| Autor: | Dmitri Simberg, William C. Trogler, Sadik C. Esener, Inanc Ortac, Jian Yang, Roger Y. Tsien, Ya san Yeh, Bradley T. Messmer |
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| Rok vydání: | 2014 |
| Předmět: |
Letter
Materials science nonhuman enzymes enzyme encapsulation Nanoparticle Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences immune response Mice Bacillus cereus Bacterial Proteins Animals General Materials Science Porosity chemistry.chemical_classification Drug Carriers Mice Inbred BALB C Nanoporous Nanoshells Mechanical Engineering technology industry and agriculture General Chemistry Penicillinase 021001 nanoscience & nanotechnology Condensed Matter Physics Small molecule Nanoshell 0104 chemical sciences Nanomedicine Enzyme chemistry silica nanoparticles 0210 nano-technology Drug carrier |
| Zdroj: | Nano Letters |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/nl404360k |
| Popis: | Although enzymes of nonhuman origin have been studied for a variety of therapeutic and diagnostic applications, their use has been limited by the immune responses generated against them. The described dual-porosity hollow nanoparticle platform obviates immune attack on nonhuman enzymes paving the way to in vivo applications including enzyme-prodrug therapies and enzymatic depletion of tumor nutrients. This platform is manufactured with a versatile, scalable, and robust fabrication method. It efficiently encapsulates macromolecular cargos filled through mesopores into a hollow interior, shielding them from antibodies and proteases once the mesopores are sealed with nanoporous material. The nanoporous shell allows small molecule diffusion allowing interaction with the large macromolecular payload in the hollow center. The approach has been validated in vivo using l-asparaginase to achieve l-asparagine depletion in the presence of neutralizing antibodies. |
| Databáze: | OpenAIRE |
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