Bran extracts from pigmented rice seeds inhibit tumor promotion in lymphoblastoid B cells by phorbol ester
| Autor: | Seok Hyun Nam, Mendel Friedman, Nobuyuki Kozukue, Hee-Jong Koh, Mi Young Kang, Sun Phil Choi |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Herpesvirus 4
Human Carcinogenicity Tests Cell Survival Biology Toxicology Flow cytometry chemistry.chemical_compound Antigen medicine Animals Anticarcinogenic Agents Humans Cytotoxicity Antigens Viral B-Lymphocytes Bran medicine.diagnostic_test Plant Extracts Reverse Transcriptase Polymerase Chain Reaction Lymphoblast food and beverages Oryza General Medicine Flow Cytometry In vitro chemistry Biochemistry Seeds Phorbol Carcinogens Tetradecanoylphorbol Acetate Tumor promotion Virus Activation Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 43(5) |
| ISSN: | 0278-6915 |
| Popis: | Using flow cytometry, we quantitatively evaluated anti-tumor-promoting activity of rice bran extracts by measuring inhibition of Epstein-Barr virus early-antigen activation (EBV-EA) induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). This assay measures anti-tumor-promoting activity and cytotoxicity of target substances using the same batch of cells. Using this short-term procedure, we have determined the anti-tumor-promoting activity of 70% ethanol-water extracts of bran (outer layer) from seeds of five pigmented rice cultivars: Jumlalocal-1, DZ 78, Elwee, LK1-3-6-12-1-1, and LK1A-2-12-1-1. The results show that, compared to an extract from the non-pigmented white cooking rice variety Chuchung, the extracts from the pigmented varieties strongly inhibited phorbol ester-induced tumor promotion in marmoset lymphoblastoid cells B95-8 in vitro. These findings may facilitate development and use of new health-promoting rice varieties. |
| Databáze: | OpenAIRE |
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