Understanding the intracellular-to-extracellular localization switch of polyhydroxybutyrate polymerase in pseudomonas backgrounds as a microevolutionary process

Autor: Carolina Berini, Nicolás Daniel Ayub, Cintia Jozefkowicz, Gabriela Soto, Margarita Stritzler
Rok vydání: 2018
Předmět:
0301 basic medicine
Statistics and Probability
030106 microbiology
General Biochemistry
Genetics and Molecular Biology
Evolution
Molecular
purl.org/becyt/ford/1 [https]
Ciencias Biológicas
03 medical and health sciences
Cytosol
Bacterial Proteins
TYPE 1 SECRETION SYSTEM (T1SS)
Pseudomonas
Gene duplication
Extracellular
Amino Acid Sequence
purl.org/becyt/ford/1.6 [https]
Gene
Phylogeny
Ciencias de las Plantas
Botánica
Polymerase
POLYHYDROXYBUTYRATE POLYMERASE (PHAC)
General Immunology and Microbiology
biology
Applied Mathematics
General Medicine
Subcellular localization
Protein subcellular localization prediction
Cell biology
Protein Transport
030104 developmental biology
Modeling and Simulation
MICROEVOLUTION
biology.protein
Extracellular Space
General Agricultural and Biological Sciences
Sequence Alignment
Acyltransferases
CIENCIAS NATURALES Y EXACTAS
Intracellular
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 0022-5193
DOI: 10.1016/j.jtbi.2018.07.039
Popis: After gene duplication, paralogous genes evolve independently, and consequently, the new proteins encoded by these duplicated genes are exposed to changes in their subcellular location. Although there are increasing evidence that phylogenetically related proteins play different functions in different subcellular compartments, the number of evolutionary steps required for the emergence of a novel protein with a novel subcellular localization remains unclear. Regarding this intriguing topic, here we examine in depth our previous reports describing both intracellular and extracellular polyhydroxybutyrate polymerases (PhaC) in the Pseudomonadales group. The recapitulation of the intracellular-to-extracellular localization switch of PhaC in these strains shows a gradual evolution from a simple cytosolic PhaC form to a complex extracellular PhaC form specifically secreted via the type 1 secretion system. This gradual evolution includes several adaptive and pre-adaptive changes at the genomic, genetic and enzymatic levels, which are intimately related to the lifestyle of organisms during the evolution of protein localization. We conclude that the protein localization switch can be an extremely complex process in nature. Fil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Berini, Carolina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Databáze: OpenAIRE
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