Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions
| Autor: | van Mameren, J., Wuite, G.J.L., Heller, I., Peterman, EJG, Wuite, GJL |
|---|---|
| Přispěvatelé: | Physics of Living Systems, LaserLaB - Molecular Biophysics, Physics and Astronomy, IoP (FNWI), Peterman, EJG, Wuite, GJL, Peterman, Erwin J. G. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Computer science Single molecule Trap stiffness calibration Optical tweezers 01 natural sciences 010309 optics 03 medical and health sciences Optics 0103 physical sciences Commercial optical tweezers Image resolution Optical trap Millisecond business.industry Force spectroscopy Time resolution Molecular motors Radiation pressure Instrument design 030104 developmental biology Biophysics DNA–protein interactions business |
| Zdroj: | Single Molecule Analysis: Methods and Protocols, 1-20 STARTPAGE=1;ENDPAGE=20;TITLE=Single Molecule Analysis Single Molecule Analysis: Methods and Protocols, 3-23 ISSUE=2nd;STARTPAGE=3;ENDPAGE=23;TITLE=Single Molecule Analysis Single Molecule Analysis ISBN: 9781617792816 ISSUE=2;STARTPAGE=3;ENDPAGE=23;TITLE=Single Molecule Analysis van Mameren, J, Wuite, G J L & Heller, I 2011, Introduction to optical tweezers: background, system designs, and commercial solutions . in EJG Peterman & GJL Wuite (eds), Single Molecule Analysis : Methods and Protocols . Methods in Molecular Biology, vol. 783, Humana Press, New York, pp. 1-20 . https://doi.org/10.1007/978-1-61779-282-3_1 Single Molecule Analysis ISBN: 9781493972708 van Mameren, J, Wuite, G J L & Heller, I 2018, Introduction to optical tweezers : Background, system designs, and commercial solutions . in E J G Peterman (ed.), Single Molecule Analysis : Methods and Protocols . 2nd edn, Methods in Molecular Biology, vol. 1665, Humana Press Inc, pp. 3-23 . https://doi.org/10.1007/978-1-4939-7271-5_1 |
| DOI: | 10.1007/978-1-4939-7271-5_1 |
| Popis: | Optical tweezers are a means to manipulate objects with light. With the technique, microscopically small objects can be held and steered while forces on the trapped objects can be accurately measured and exerted. Optical tweezers can typically obtain a nanometer spatial resolution, a piconewton force resolution, and a millisecond time resolution, which make them excellently suited to study biological processes from the single-cell down to the single-molecule level. In this chapter, we provide an introduction on the use of optical tweezers in single-molecule approaches. We introduce the basic principles and methodology involved in optical trapping, force calibration, and force measurements. Next, we describe the components of an optical tweezers setup and their experimental relevance in single-molecule approaches. Finally, we provide a concise overview of commercial optical tweezers systems. Commercial systems are becoming increasingly available and provide access to single-molecule optical tweezers experiments without the need for a thorough background in physics. © Springer Science+Business Media, LLC 2011. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|