Vitamin D3and Calcipotriol Enhance the Secretion of Transforming Growth Factor-β1 and -β2 in Cultured Murine Keratinocytes
Autor: | Katri Koli, Jorma Keski-Oja |
---|---|
Rok vydání: | 1993 |
Předmět: |
DNA Replication
Keratinocytes Vitamin medicine.medical_specialty Time Factors Clinical Biochemistry Tritium Cell Line Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Calcitriol Neutralization Tests Transforming Growth Factor beta biology.animal Internal medicine Vitamin D and neurology medicine Animals Secretion RNA Messenger Mink Calcipotriol Cells Cultured 030304 developmental biology Mice Inbred BALB C 0303 health sciences biology Cell Biology Transforming growth factor beta Blotting Northern Molecular biology 3. Good health Kinetics medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis biology.protein Growth inhibition Keratinocyte Cell Division Thymidine |
Zdroj: | Growth Factors. 8:153-163 |
ISSN: | 1029-2292 0897-7194 |
Popis: | Vitamin D3 and its analogue calcipotriol (MC 903) inhibit the proliferation of cultured keratinocytes and induce their differentiation. Since TGF beta s are very potent inhibitors of keratinocyte growth we studied the effects of vitamin D3 and calcipotriol on the secretion of TGF beta in cultured murine keratinocytes. Vitamin D3 and calcipotriol (10(-6)-10(-9) M) inhibited the DNA-synthesis of mouse keratinocytes by 50-80% in a time and dose-dependent manner as measured by [3H]-thymidine incorporation. Analysis of the conditioned medium of the keratinocytes indicated that the cells secreted into their medium activity that inhibited the growth of indicator Mv1Lu mink lung epithelial cells. Neutralizing antibodies against TGF beta 1 and TGF beta 2 decreased, and when used together, prevented the observed growth inhibition of the indicator cells. Heat treatment of the conditioned medium, which activates latent forms of TGF beta, revealed higher levels of growth inhibitory activity in the medium from vitamin D3 and calcipotriol treated than from control cultures indicating that a fraction of TGF beta was in a latent form. Active TGF beta was, however, detected considerably more in vitamin D3 and calcipotriol treated cultures than in control cultures. Immunoblotting analysis of the medium revealed enhanced secretion of TGF beta protein. These results indicate that enhanced TGF beta 1 and TGF beta 2 secretion and activity is associated with vitamin D3-mediated growth inhibition of cultured keratinocytes. |
Databáze: | OpenAIRE |
Externí odkaz: |