Genetic profiling of Gleason grade 4/5 prostate cancer: which is the best prostatic control tissue?
| Autor: | Mitchell C. Caldwell, Mamatha Mahadevappa, Thomas A. Stamey, Zhaomei Zhang, John E. McNEAL, Rosalie Nolley, Janet A. Warrington, Zhenbin Fan, Zuxiong Chen |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Central Zone Pathology medicine.medical_specialty Microarray Urology medicine.medical_treatment Prostatic Hyperplasia Down-Regulation Polymerase Chain Reaction Prostate cancer Prostate Gene expression Medicine Humans business.industry Prostatectomy Gene Expression Profiling Prostatic Neoplasms Hyperplasia medicine.disease language.human_language Up-Regulation medicine.anatomical_structure Genetic marker language business |
| Zdroj: | The Journal of urology. 170(6 Pt 1) |
| ISSN: | 0022-5347 |
| Popis: | We examined the variation in gene expression profiles of prostate cancer caused by zone specific genes.Ten normal central zone, 10 transition zone (benign prostatic hyperplasia) and 6 normal peripheral zone tissues from radical retropubic prostatectomies were compared to each other and to 12 peripheral zone Gleason grade 4/5 cancers. Test chips and HuGeneFL6800 (Affymetrix, Inc., Santa Clara, California) chips were used to assay the transcribed genes. Data were obtained with the Microarray Suite Version 4.0.1 (Affymetrix, Inc.) and analyzed statistically.Substantially different gene expression profiles were found depending upon which of the 3 zonal tissues were used as a control. All 3 profiles were compared for efficiency (ability to locate genes) and for robustness (the magnitude of difference between the control and the Gleason grade 4/5 tissue). Microscopically normal appearing peripheral zone tissue at the gene level shows many characteristics of Gleason grade 4/5 cancer.Gene expression profiles of prostate cancer are affected by the zonal location of the control tissue and the cancer. |
| Databáze: | OpenAIRE |
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