Protective effects of protopine on hydrogen peroxide-induced oxidative injury of PC12 cells via Ca2+ antagonism and antioxidant mechanisms
| Autor: | Xianghua Xiao, Hua Yang, Chaoyun Wang, Xiu-Ping Zhu, Yuan-Hui Zhang, Juntian Liu, Jingwen Hu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Programmed cell death
Cell Survival Berberine Alkaloids Apoptosis Pharmacology medicine.disease_cause PC12 Cells Neuroprotection Antioxidants Superoxide dismutase Lipid peroxidation chemistry.chemical_compound medicine Animals Benzophenanthridines Membrane Potential Mitochondrial chemistry.chemical_classification biology Caspase 3 Glutathione peroxidase Hydrogen Peroxide Oxidants Rats Oxidative Stress Gene Expression Regulation chemistry Biochemistry Catalase biology.protein Protopine Calcium Lipid Peroxidation Oxidative stress |
| Zdroj: | European Journal of Pharmacology. 591:21-27 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2008.06.045 |
| Popis: | Calcium and lipid peroxidation play important roles in oxidative stress-induced cellular injury and apoptosis, which ultimately cause cell death. In this study we examined whether protopine had a neuroprotection against H(2)O(2)-induced injury in PC12 cells. Pretreatment of PC12 cells with protopine improved the cell viability, enhanced activities of superoxide dismutase, glutathione peroxidase and catalase, and decreased malondialdehyde level in the H(2)O(2) injured cells. Protopine also reversed the increased intracellular Ca(2+) concentration and the reduced mitochondrial membrane potential caused by H(2)O(2) in the cells. Furthermore, protopine was able to inhibit caspase-3 expression and cell apoptosis induced by H(2)O(2). In summary, this study demonstrates that protopine is able to relieve H(2)O(2)-induced oxidative stress and apoptosis in PC12 cells, at least in part, by Ca(2+) antagonism and antioxidant mechanisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect